Benard J, Da Silva J, Riou G
Eur J Cancer Clin Oncol. 1983 Jan;19(1):65-72. doi: 10.1016/0277-5379(83)90400-5.
Cells obtained from human solid tumor biopsies and from ascites of various histological origin were cultured in soft agar. Forty-six out of 72 malignant biopsy specimens, consisting mainly of ovarian and breast adenocarcinoma, were grown successfully. Cloning efficiencies ranged from 0.001 to 1%. In a few cases large variations occurred in the different specimens from the same patient. The cells in the colonies had the same morphological characteristics as those of the original tumor. The soft agar assay was used to study the chemotherapeutic response of ovarian tumors.
从人类实体瘤活检组织以及来自各种组织学来源的腹水中获取的细胞，在软琼脂中进行培养。72个恶性活检标本中有46个成功生长，这些标本主要由卵巢和乳腺腺癌组成。克隆效率范围为0.001%至1%。在少数情况下，同一患者的不同标本出现了较大差异。克隆中的细胞与原始肿瘤细胞具有相同的形态特征。软琼脂试验用于研究卵巢肿瘤的化疗反应。
