Loh W, Woodcock B G
Arzneimittelforschung. 1983;33(4):568-70.
For the quantitative and qualitative determination of dihydroergotoxine methanesulphonate (DCCK) a radioimmunoassay was developed. This method involves use of an antibody against the dihydrolysergic acid moiety and an 125I-labelled dihydrolysergic tracer. The differentiation of dihydroergopeptides, lysergic acid and the ergopeptides was obtained by modification of extraction methods already published. The assay procedure detects around 10 pg/ml dihydroergotoxine. Dihydrolysergic acid and lysergic acid are not detectable up to about 6 ng/ml. Ergometrine and alpha-ergocryptine show cross-reactivities of 14% and 7.5%, respectively. All dihydroergopeptides included in dihydroergotoxine show the same cross-reactivity as dihydroergotoxine itself. Intra- and inter-assay variations were approximately 12% and 13%, respectively.
为了对甲磺酸双氢麦角毒碱（DCCK）进行定量和定性测定，开发了一种放射免疫分析法。该方法涉及使用针对双氢麦角酸部分的抗体和125I标记的双氢麦角酸示踪剂。通过改进已发表的提取方法实现了双氢麦角肽、麦角酸和麦角肽的区分。该测定程序可检测到约10 pg/ml的双氢麦角毒碱。双氢麦角酸和麦角酸在约6 ng/ml以下无法检测到。麦角新碱和α-麦角隐亭的交叉反应率分别为14%和7.5%。双氢麦角毒碱中包含的所有双氢麦角肽与双氢麦角毒碱本身具有相同的交叉反应性。批内和批间变异分别约为12%和13%。
