Collignon F, Pradelles P
Eur J Nucl Med. 1984;9(1):23-7. doi: 10.1007/BF00254345.
The development of three analogous radioimmunoassay (RIA) procedures for dihydroergotoxine components is described. The antisera were produced by immunization of rabbits with immunogens obtained by coupling egg albumin to the indole group of each ergot alkaloid derivative. In each radioimmunoassay, antibodies do not cross-react more than 5% with the two other derivatives. The tracers iodinated with iodine 125 were prepared by the chloramine-T method and purified by thin layer chromatography. Both antibody affinity and high specific radioactivity of tracers allow a sensitive assay (detection limit less than 20 pg/ml) in human plasma. After high performance liquid chromatography of extracted plasma, immunoreactive materials other than those corresponding to the elution of the three dihydroergotoxine components were not detected. Two preliminary pharmacokinetic profiles obtained in dog and human for each derivative are shown.
本文描述了三种用于二氢麦角隐亭成分的类似放射免疫分析（RIA）方法的开发。抗血清是通过用免疫原免疫兔子制备的，免疫原是通过将卵清蛋白与每种麦角生物碱衍生物的吲哚基团偶联而获得的。在每种放射免疫分析中，抗体与其他两种衍生物的交叉反应不超过5%。用氯胺-T法制备了用碘125标记的示踪剂，并通过薄层色谱法进行纯化。抗体亲和力和示踪剂的高比放射性使得能够在人血浆中进行灵敏的分析（检测限小于20 pg/ml）。对提取的血浆进行高效液相色谱分析后，未检测到除对应于三种二氢麦角隐亭成分洗脱的物质以外的免疫反应性物质。展示了每种衍生物在狗和人中获得的两个初步药代动力学概况。
