放射受体分析法与放射免疫分析法在兔血浆样品中二氢麦角毒碱测定中的比较。

Comparison between radioreceptor assay and radioimmunoassay for the determination of dihydroergotoxine in rabbit plasma samples.

作者信息

Shimada N, Ushioda K, Nagatsuka S, Ueda T, Yokoshima T

出版信息

J Immunol Methods. 1983 Dec 16;65(1-2):191-8. doi: 10.1016/0022-1759(83)90315-0.

Abstract

Two analytical methods, radioreceptor assay and radioimmunoassay, for the determination of dihydroergotoxine have been developed. Antiserum, providing sufficient sensitivity for the radioimmunoassay, was produced by immunizing rabbits with D-lysergic acid coupled to bovine serum albumin. Radioreceptor assay utilizing dopamine receptor was carried out to determine dihydroergotoxine and its pharmacologically active metabolites in rabbit plasma, and the result was compared with that obtained by radioimmunoassay. The values obtained in both assays were almost identical; it was, therefore, assumed that the plasma concentrations of dihydroergotoxine determined by the present radioimmunoassay reflects the amount of unchanged drug and its active metabolites.

摘要

已开发出两种用于测定双氢麦角毒碱的分析方法，即放射受体分析法和放射免疫分析法。通过用与牛血清白蛋白偶联的D-麦角酸免疫兔子来制备对放射免疫分析具有足够灵敏度的抗血清。利用多巴胺受体进行放射受体分析，以测定兔血浆中的双氢麦角毒碱及其药理活性代谢物，并将结果与放射免疫分析所得结果进行比较。两种分析方法得到的值几乎相同；因此，可以假定通过本放射免疫分析测定的双氢麦角毒碱的血浆浓度反映了未变化药物及其活性代谢物的量。