Lambert R D
Rev Can Biol Exp. 1983 Mar;42(1):63-6.
Different concentrations of rabbit or bovine follicular fluid were added to a defined medium or to rabbit oestrous oviduct fluid containing 13 hours in utero incubated sperm. With increasing concentration of follicular fluid, the percentage of fertilization was progressively and significantly lower than in oestrous tubal secretions or Brackett's medium, alone or containing up to 5% of follicular fluid. Utilization of ova, with or without cumulus clot, to the fertilization media, had no significant effect on fertilization of rabbit eggs. Both kinds of follicular fluid used did inhibit the motility of capacitated sperm.
将不同浓度的兔或牛卵泡液添加到特定培养基中，或添加到含有在子宫内孵育13小时精子的兔发情期输卵管液中。随着卵泡液浓度的增加，受精率逐渐显著低于发情期输卵管分泌物或布雷克特培养基单独使用时或含有高达5%卵泡液时的受精率。有无卵丘凝块的卵子对受精培养基的利用，对兔卵受精没有显著影响。所使用的两种卵泡液均抑制了获能精子的活力。
