Lawson L D, Hill E G, Holman R T
J Nutr. 1983 Sep;113(9):1827-35. doi: 10.1093/jn/113.9.1827.
The influence of dietary isomeric cis and trans octadecenoic fatty acids (ICTO) on the metabolism of polyunsaturated fatty acids in rat tissues was studied by feeding a defined diet rich in partially hydrogenated soybean oil. Experimental and control animals received equal and more than adequate amounts of linoleic and linolenic acids. The total phospholipid (PL) fatty acids of liver, heart, testis, brain and sciatic nerve, and the fatty acids of liver triacylglyceride, cholesteryl ester and individual PL classes were analyzed by capillary gas chromatography. The content of arachidonic acid in the total fatty acids of liver lipids of ICTO-fed rats was found to be lower than those from control rats by the following amounts (in percent): total PL, 35; phosphatidylcholine, 50; phosphatidylinositol, 35; phosphatidylserine 26; phosphatidylethanolamine, 12; cholesteryl ester, 55; and tryglyceride 75. PL 18:2 omega 6 and 20:3 omega 6 levels were elevated, suggesting inhibition of the desaturase-elongase enzymes involved in synthesis of arachidonic acid. Synthesis of 20:5 omega 3 and 20:3 omega 9 was accentuated by the ICTO diet, suggesting an omega 6 series-specific inhibition. A nearly perfect negative correlation between cis 12-18:1 and arachidonic acid was found in liver PL.
通过喂食富含部分氢化大豆油的特定饮食，研究了膳食中顺式和反式十八碳烯酸异构体（ICTO）对大鼠组织中多不饱和脂肪酸代谢的影响。实验动物和对照动物摄入了等量且充足的亚油酸和亚麻酸。采用毛细管气相色谱法分析了肝脏、心脏、睾丸、大脑和坐骨神经中的总磷脂（PL）脂肪酸，以及肝脏甘油三酯、胆固醇酯和各PL类别的脂肪酸。发现喂食ICTO的大鼠肝脏脂质总脂肪酸中花生四烯酸的含量比对照大鼠低以下幅度（百分比）：总PL，35；磷脂酰胆碱，50；磷脂酰肌醇，35；磷脂酰丝氨酸，26；磷脂酰乙醇胺，12；胆固醇酯，55；甘油三酯，75。PL 18:2 ω6和20:3 ω6水平升高，表明参与花生四烯酸合成的去饱和酶-延长酶受到抑制。ICTO饮食增强了20:5 ω3和20:3 ω9的合成，表明存在ω6系列特异性抑制。在肝脏PL中发现顺式12-18:1与花生四烯酸之间存在近乎完美的负相关。
