从沙漠蝗发育中的卵和埃及棉叶夜蛾蛹中分离出蜕皮甾酸和20-羟基蜕皮甾酸的鉴定。

Identification of ecdysonoic acid and 20-hydroxyecdysonoic acid isolated from developing eggs of Schistocerca gregaria and pupae of Spodoptera littoralis.

作者信息

Isaac R E, Milner N P, Rees H H

出版信息

Biochem J. 1983 Jul 1;213(1):261-5. doi: 10.1042/bj2130261.

DOI:10.1042/bj2130261

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152117/

Abstract

Ecdysonoic acid and 20-hydroxyecdysonoic acid have been purified from developing eggs of the desert locust, Schistocerca gregaria, by high performance liquid chromatography (h.p.l.c.), and their structures were determined by p.m.r. spectroscopy and fast atom bombardment mass spectrometry of the free and methyl ester derivatives. 20-Hydroxyecdysonoic acid was also characterized from Spodoptera littoralis pupae. The occurrence of both 20-hydroxyecdysonoic acid and ecdysonoic acid in Sp. littoralis pupae was also established by h.p.l.c. comparison of the 3H-labelled acids formed from [3H]ecdysone and of their methyl esters with the corresponding substances from Sch. gregaria. The significance of ecdysteroid acids as products of ecdysteroid inactivation is discussed.

摘要

通过高效液相色谱法（h.p.l.c.）从沙漠蝗虫（Schistocerca gregaria）发育中的卵中纯化出蜕皮甾酮酸和20-羟基蜕皮甾酮酸，并通过对游离及甲酯衍生物进行核磁共振光谱法（p.m.r.）和快原子轰击质谱法确定了它们的结构。还从滨海灰翅夜蛾（Spodoptera littoralis）蛹中鉴定出了20-羟基蜕皮甾酮酸。通过高效液相色谱法比较由[3H]蜕皮激素形成的3H标记酸及其甲酯与来自沙漠蝗虫相应物质，也确定了滨海灰翅夜蛾蛹中同时存在20-羟基蜕皮甾酮酸和蜕皮甾酮酸。讨论了蜕皮甾酮酸作为蜕皮甾酮失活产物的意义。

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2

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3

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本文引用的文献

1

Biosynthesis and inactivation of ecdysone during the pupal-adult development of the cabbage butterfly, Pieris brassicae L.

Steroids. 1981 Dec;38(6):633-50. doi: 10.1016/0039-128x(81)90083-0.

2

Sulphage esters as inactivation products of ecdysone in Locusta migratoria.

Hoppe Seylers Z Physiol Chem. 1973 Sep;354(9):1043-8. doi: 10.1515/bchm2.1973.354.2.1043.

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