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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Greenwood D R, Rees H H

Biochem J. 1982 Dec 15;208(3):857-64. doi: 10.1042/bj2080857.

The fates of the 22-pro-R and 22-pro-S hydrogen atoms of cholesterol during the biosynthesis of ecdysteroids in the ovaries of Schistocerca gregaria were investigated. 2. Two stereospecifically labelled cholesterol species, obtained by incubating 3R,2R- and 3R,2S-[2-14C, 2-3H]mevalonic acid with rat liver preparations, were administered, in turn, to maturing adult female locusts and the radiolabelled ecdysteroid conjugates isolated from the eggs. Enzymic hydrolysis of the conjugates yielded free ecdysteroids, from which ecdysone was purified. 3. Derivative formation and oxidation at C-22 of both ecdysone samples indicated that the 22-pro-R and 22-pro-S hydrogen atoms of cholesterol were stereospecifically eliminated and retained respectively during ecdysteroid formation. This indicates that C-22 hydroxylation in ecdysone biosynthesis is direct and occurs with retention of configuration.

研究了在沙漠蝗卵巢中蜕皮甾体生物合成过程中胆固醇22 - 前R和22 - 前S氢原子的命运。2. 通过将3R,2R - 和3R,2S - [2 - ¹⁴C, 2 - ³H]甲羟戊酸与大鼠肝脏制剂孵育获得的两种立体特异性标记的胆固醇，依次给予成熟的成年雌性蝗虫，并从卵中分离出放射性标记的蜕皮甾体缀合物。缀合物的酶促水解产生游离的蜕皮甾体，从中纯化出蜕皮激素。3. 两种蜕皮激素样品在C - 22处的衍生物形成和氧化表明，在蜕皮甾体形成过程中，胆固醇的22 - 前R和22 - 前S氢原子分别被立体特异性消除和保留。这表明蜕皮激素生物合成中的C - 22羟基化是直接的，并且构型保持不变。

Greenwood D R, Rees H H

Biochem J. 1982 Dec 15;208(3):857-64. doi: 10.1042/bj2080857.

The fates of the 22-pro-R and 22-pro-S hydrogen atoms of cholesterol during the biosynthesis of ecdysteroids in the ovaries of Schistocerca gregaria were investigated. 2. Two stereospecifically labelled cholesterol species, obtained by incubating 3R,2R- and 3R,2S-[2-14C, 2-3H]mevalonic acid with rat liver preparations, were administered, in turn, to maturing adult female locusts and the radiolabelled ecdysteroid conjugates isolated from the eggs. Enzymic hydrolysis of the conjugates yielded free ecdysteroids, from which ecdysone was purified. 3. Derivative formation and oxidation at C-22 of both ecdysone samples indicated that the 22-pro-R and 22-pro-S hydrogen atoms of cholesterol were stereospecifically eliminated and retained respectively during ecdysteroid formation. This indicates that C-22 hydroxylation in ecdysone biosynthesis is direct and occurs with retention of configuration.

研究了在沙漠蝗卵巢中蜕皮甾体生物合成过程中胆固醇22 - 前R和22 - 前S氢原子的命运。2. 通过将3R,2R - 和3R,2S - [2 - ¹⁴C, 2 - ³H]甲羟戊酸与大鼠肝脏制剂孵育获得的两种立体特异性标记的胆固醇，依次给予成熟的成年雌性蝗虫，并从卵中分离出放射性标记的蜕皮甾体缀合物。缀合物的酶促水解产生游离的蜕皮甾体，从中纯化出蜕皮激素。3. 两种蜕皮激素样品在C - 22处的衍生物形成和氧化表明，在蜕皮甾体形成过程中，胆固醇的22 - 前R和22 - 前S氢原子分别被立体特异性消除和保留。这表明蜕皮激素生物合成中的C - 22羟基化是直接的，并且构型保持不变。