DeLeon D V, Cox K H, Angerer L M, Angerer R C
Dev Biol. 1983 Nov;100(1):197-206. doi: 10.1016/0012-1606(83)90211-7.
Previous studies demonstrated that the pronucleus of the unfertilized sea urchin egg contains a high concentration of transcripts complementary to the early histone repeat unit (D. L. Venezky, L. M. Angerer, and R. C. Angerer (1981). Cell 24, 385-391.) In this paper, in situ hybridization techniques of improved sensitivity are used to show that these nuclear RNAs include authentic histone mRNA but not spacer-complementary sequences. It is estimated that most early-variant histone mRNA contained in the egg is, in fact, restricted to the pronucleus. These mRNAs are released to the cytoplasm at the time of nuclear breakdown of first cleavage and rapidly distribute throughout the cytoplasm.
先前的研究表明，未受精的海胆卵原核中含有高浓度的与早期组蛋白重复单元互补的转录本（D. L. 韦内斯基、L. M. 安热尔和R. C. 安热尔（1981年）。《细胞》24卷，第385 - 391页）。在本文中，使用了灵敏度更高的原位杂交技术来表明这些核RNA包括真实的组蛋白mRNA，但不包括间隔区互补序列。据估计，卵中所含的大多数早期变体组蛋白mRNA实际上局限于原核。这些mRNA在第一次卵裂核破裂时释放到细胞质中，并迅速分布于整个细胞质。
