Creason G L, Holowach L P, Thompson J F, Madison J T
Biochem Biophys Res Commun. 1983 Dec 28;117(3):658-62. doi: 10.1016/0006-291x(83)91647-9.
In vitro translation experiments indicate that absence of the beta-subunit of 7S storage protein in soybean (Glycine max L. Merr. cv. Provar) cotyledons cultured on methionine-supplemented medium is due to lack of functional mRNA for that polypeptide. Relative amounts of functional mRNA for the 7S alpha'- and alpha-subunits were unaffected by methionine in the cotyledon culture medium. Measurements of beta-subunit accumulation in cotyledons transferred from basal medium to methionine-supplemented medium show that methionine inhibits continued accumulation of the beta-subunit after synthesis of the beta-subunit has begun, and that methionine does not promote degradation of existing beta-subunit.
体外翻译实验表明，在添加蛋氨酸的培养基上培养的大豆（Glycine max L. Merr. cv. Provar）子叶中，7S贮藏蛋白β亚基的缺失是由于缺乏该多肽的功能性mRNA。子叶培养基中的蛋氨酸不影响7Sα'-亚基和α-亚基功能性mRNA的相对含量。对从基础培养基转移到添加蛋氨酸培养基的子叶中β亚基积累情况的测量表明，蛋氨酸在β亚基合成开始后抑制其持续积累，且蛋氨酸不会促进现有β亚基的降解。
