冠状病毒SD和SK与鼠冠状病毒MHV-A59具有广泛的核苷酸同源性，比人和鼠冠状病毒之间的同源性还要高。
Coronaviruses SD and SK share extensive nucleotide homology with murine coronavirus MHV-A59, more than that shared between human and murine coronaviruses.
作者信息
Weiss S R
出版信息
Virology. 1983 Apr 30;126(2):669-77. doi: 10.1016/s0042-6822(83)80022-1.
Abstract
A cDNA probe representing the genome of mouse hepatitis virus (MHV) strain A59 (MHV-A59) was used to measure nucleotide sequence homologies among murine and human coronaviruses and the SD and SK coronaviruses isolated by Burks et al. Since SD and SK were isolated by inoculation of multiple sclerosis (MS) central nervous system (CNS) tissue into mice or cultured mouse cells, it is important to determine their relationships to other murine and human coronavirus isolates. Our results indicate that SD and SK share almost complete nucleotide homology (approximately 90%) with MHV-A59 and generate subgenomic RNAs of the same sizes as MHV-A59. The human coronavirus (HCV) strains tested show less homology with MHV-A59. The immunologically unrelated HCV-229E shows no nucleotide homology with MHV-A59. The immunologically cross-reactive HCV-OC43 shows nucleotide homology with MHV-A59 by blot hybridization but not when hybridized in solution and assayed by S1 nuclease digestion.
摘要
用代表小鼠肝炎病毒（MHV）A59株（MHV-A59）基因组的cDNA探针来测定鼠源和人源冠状病毒以及Burks等人分离的SD和SK冠状病毒之间的核苷酸序列同源性。由于SD和SK是通过将多发性硬化症（MS）中枢神经系统（CNS）组织接种到小鼠或培养的小鼠细胞中分离得到的，因此确定它们与其他鼠源和人源冠状病毒分离株之间的关系很重要。我们的结果表明，SD和SK与MHV-A59几乎具有完全的核苷酸同源性（约90%），并产生与MHV-A59大小相同的亚基因组RNA。所测试的人冠状病毒（HCV）株与MHV-A59的同源性较低。免疫上不相关的HCV-229E与MHV-A59没有核苷酸同源性。免疫交叉反应的HCV-OC43通过印迹杂交显示与MHV-A59有核苷酸同源性，但在溶液中杂交并用S1核酸酶消化进行检测时则没有。
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