人冠状病毒229E和OC43对人神经细胞原代培养物的感染。
Infection of primary cultures of human neural cells by human coronaviruses 229E and OC43.
作者信息
Bonavia A, Arbour N, Yong V W, Talbot P J
机构信息
Laboratory of Neuroimmunovirology, University of Quebec, Laval, Canada.
出版信息
J Virol. 1997 Jan;71(1):800-6. doi: 10.1128/JVI.71.1.800-806.1997.
Abstract
We evaluated the ability of human coronaviruses to infect primary cultures of human neural cells. Double immunofluorescence with antibodies to virus and cell markers showed infection of fetal astrocytes and of adult microglia and astrocytes by strain OC43. RNA amplification revealed infection of fetal astrocytes, adult microglia, and a mixed culture of adult oligodendrocytes and astrocytes by strain 229E. Infectious virus was released only from fetal astrocytes, with higher titers for OC43. Human coronaviruses have the capacity to infect some cells of the central nervous system, although infection of adult cells appears abortive.
摘要
我们评估了人类冠状病毒感染人神经细胞原代培养物的能力。用病毒抗体和细胞标志物进行的双重免疫荧光显示，OC43毒株可感染胎儿星形胶质细胞、成人小胶质细胞和星形胶质细胞。RNA扩增显示，229E毒株可感染胎儿星形胶质细胞、成人小胶质细胞以及成人少突胶质细胞和星形胶质细胞的混合培养物。仅胎儿星形胶质细胞释放出传染性病毒，OC43毒株的滴度更高。人类冠状病毒有能力感染中枢神经系统的一些细胞，尽管对成人细胞的感染似乎是流产性的。
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