Rebut-Bonneton C, Demignon J, Amor B, Miravet L
J Endocrinol. 1983 Dec;99(3):347-53. doi: 10.1677/joe.0.0990347.
Fetal bone resorption was measured by an organ culture technique using fetuses from intact or thyroparathyroidectomized pregnant rats. These experiments were performed to investigate the effects of 1,25-dihydroxycholecalciferol (1,25-DHCC) and salmon calcitonin (SCT) in pregnant rats, on both fetal growth and fetal bone resorption. Pregnant rats were given 0.1-0.5 microgram 1,25-DHCC per day from day 17 of gestation: in intact rats bone resorption was increased and fetal growth decreased; 1,25-DHCC probably modified fetal bone resorption in the absence of fetal parathyroid secretion. Infusion of SCT in minipumps (30 mu./h) did not modify plasma calcium levels in either the mother or fetuses, neither was bone resorption altered. In 1,25-DHCC-treated rats, SCT infusion resulted in an increase in fetal weight and a decrease in fetal bone resorption. On the other hand, SCT infusion was found to facilitate phosphate accumulation in fetuses. At the end of the SCT infusion the SCT concentration was 450 ng/l in maternal plasma and 553 +/- 60 ng/l in fetal plasma. Salmon calcitonin was shown to cross the placental barrier in the rats; it may interact with the effects of 1,25-DHCC in the fetus.
采用器官培养技术，利用来自完整或甲状旁腺切除的妊娠大鼠的胎儿，测量胎儿骨吸收情况。进行这些实验是为了研究1,25 - 二羟胆钙化醇（1,25 - DHCC）和鲑鱼降钙素（SCT）对妊娠大鼠胎儿生长和胎儿骨吸收的影响。从妊娠第17天起，每天给妊娠大鼠注射0.1 - 0.5微克1,25 - DHCC：在完整大鼠中，骨吸收增加而胎儿生长减少；1,25 - DHCC可能在胎儿甲状旁腺分泌缺乏的情况下改变胎儿骨吸收。用微型泵以30微升/小时的速度输注SCT，对母体或胎儿的血浆钙水平均无影响，骨吸收也未改变。在接受1,25 - DHCC治疗的大鼠中，输注SCT导致胎儿体重增加，胎儿骨吸收减少。另一方面，发现输注SCT有助于胎儿体内磷酸盐的积累。在输注SCT结束时，母体血浆中SCT浓度为450纳克/升，胎儿血浆中为553±60纳克/升。结果表明，鲑鱼降钙素在大鼠中可穿过胎盘屏障；它可能与1,25 - DHCC在胎儿中的作用相互影响。
