Al-Shawi A, Dawnay A, Landon J
Clin Chem. 1984 Jan;30(1):38-41.
Using human liver ferritin as a model, we have developed a two-site immunoradiometric assay. Sample (or standard) is incubated with excess rabbit antiserum and 125I-labeled specific sheep antibodies directed against different antigenic determinants until equilibrium is reached. Then excess sheep anti-rabbit immunoglobulin G serum, covalently linked to magnetizable particles, is added. The radioactivity bound to these particles is directly proportional to the amount of ferritin present. The assay is reproducible and precise throughout the clinically important range for ferritin in serum. Late addition of second antibodies coupled to magnetizable particles is a novel, simple, rapid, and universally-applicable separation technique for two-site immunoradiometric assays. By this approach, the first immunological reaction can occur in liquid phase, which shortens the reaction time, maximizes sensitivity, and avoids the need for continuous mixing, as compared with methods in which one of the first antibodies is immobilized on a solid phase.
以人肝铁蛋白为模型，我们开发了一种双位点免疫放射分析方法。将样品（或标准品）与过量的兔抗血清以及针对不同抗原决定簇的125I标记的特异性羊抗体一起孵育，直至达到平衡。然后加入与可磁化颗粒共价连接的过量羊抗兔免疫球蛋白G血清。与这些颗粒结合的放射性与存在的铁蛋白量成正比。在血清中铁蛋白的临床重要范围内，该分析方法具有可重复性和精确性。后期加入与可磁化颗粒偶联的二抗是一种用于双位点免疫放射分析的新颖、简单、快速且普遍适用的分离技术。通过这种方法，第一次免疫反应可以在液相中发生，与其中一种第一抗体固定在固相上的方法相比，这缩短了反应时间，使灵敏度最大化，并避免了持续混合的需要。
