Padrós E, Duñach M, Sabés M
Biochim Biophys Acta. 1984 Jan 11;769(1):1-7. doi: 10.1016/0005-2736(84)90002-6.
The effects produced on bacteriorhodopsin by low concentrations of several detergents have been studied by absorption and fourth-derivative spectrophotometry. Sodium dodecyl sulfate induces the appearance of the blue form of bacteriorhodopsin (lambda max = 600 nm) at pH values up to 7.0 in a reversible manner. The apparent pK of the purple-to-blue transition raised with increasing concentration of SDS. Of the other detergents tested, only sodium dodecyl-N-sarcosinate showed a slight red-shift of the absorption band to 580 nm, whereas sodium taurocholate, Triton X-100 and cetyltrimethylammonium bromide did not favour the appearance of the blue form. The effect of SDS was found to be consistent with a localized conformational change that moves away the counter-ion of the protonated Schiff base.
通过吸收光谱法和四阶导数分光光度法研究了几种低浓度去污剂对细菌视紫红质产生的影响。在pH值高达7.0的条件下，十二烷基硫酸钠以可逆的方式诱导细菌视紫红质蓝色形式（最大吸收波长=600nm）的出现。随着SDS浓度的增加，紫色到蓝色转变的表观pK升高。在所测试的其他去污剂中，只有十二烷基-N-肌氨酸钠显示出吸收带轻微红移至580nm，而牛磺胆酸钠、Triton X-100和十六烷基三甲基溴化铵则不利于蓝色形式的出现。发现SDS的作用与一种局部构象变化一致，该变化使质子化席夫碱的抗衡离子远离。
