Ohsawa T, Nakane-Hikichi K, Nagai Y
Biochim Biophys Acta. 1984 Jan 17;792(1):79-83.
Serially cultured human diploid fibroblasts have a finite lifetime in vitro, and this phenomenon was postulated as cellular aging. Neutral glycosphingolipids (GSLs) from human fetal lung-derived diploid fibroblast cell lines, WI-38 and TIG-1 cells, were studied during cellular aging. Both cell lines had at least four types of neutral GSLs. It was found that neutral GSLs changed with aging, the most conspicuous alteration being a 2-4-fold increase in the content of ceramide monohexoside. This change was invariably observed in either WI-38 or TIG-1 cells.
连续培养的人二倍体成纤维细胞在体外具有有限的寿命，这种现象被认为是细胞衰老。在细胞衰老过程中，对源自人胎儿肺的二倍体成纤维细胞系WI-38和TIG-1细胞的中性糖鞘脂（GSL）进行了研究。这两种细胞系都至少有四种类型的中性GSL。研究发现，中性GSL随衰老而变化，最显著的变化是单己糖神经酰胺含量增加2至4倍。在WI-38或TIG-1细胞中均始终观察到这种变化。
