Stein W, Bohner J
Clin Chem. 1984 Feb;30(2):238-42.
During the last three years we and other have observed discrepancies between results for creatine kinase isoenzyme MB as measured with the mechanized ion-exchange chromatographic method in the Du Pont aca and those by other techniques. These observations prompted us to investigate the influence of the matrix on the Du Pont CK-MB assay. We conclude that, apart from possible interferences by CK-MM, CK-BB, and both types of macro CK, the aca will give apparent CK-MB activities that are directly related to protein concentration and inversely related to sodium chloride concentration. Practical consequences for the routine and emergency laboratories are: no diluted samples are allowed; application is restricted to samples from patients suspected of acute myocardial infarction which show upper-normal total CK activity; and multiple timed samples are run, in order to recognize the typical change in enzyme pattern with time.
在过去三年中，我们和其他人观察到，用杜邦aca的机械化离子交换色谱法测定的肌酸激酶同工酶MB结果与其他技术测定的结果之间存在差异。这些观察结果促使我们研究基质对杜邦CK-MB测定的影响。我们得出结论，除了CK-MM、CK-BB和两种类型的巨CK可能产生干扰外，aca会给出明显的CK-MB活性，该活性与蛋白质浓度直接相关，与氯化钠浓度呈负相关。对常规和急诊实验室的实际影响是：不允许稀释样本；该方法仅限于应用于怀疑患有急性心肌梗死且总CK活性高于正常上限的患者样本；并且要进行多次定时样本检测，以便识别酶谱随时间的典型变化。
