人血浆中胰高血糖素灵敏且特异的放射免疫分析方法的评估及其临床应用。

Evaluation of a sensitive and specific radioimmunoassay for pancreatic glucagon in human plasma and its clinical application.

作者信息

Borghi V C, Wajchenberg B L, Albuquerque R H

出版信息

Clin Chim Acta. 1984 Jan 16;136(1):39-48. doi: 10.1016/0009-8981(84)90245-6.

Abstract

A glucagon radioimmunoassay employing antiserum specific for pancreatic glucagon is described. Glucagon was radioiodinated by the chloramine T technique and purified on QAE-Sephadex A 25 to a specific activity of 225 mu Ci/microgram. The standard curve allowed measurements from 12 to 500 pg/ml with sensitivity of 17.5 pg/ml, precision of 6.3-14.9% (CV, within-assay) and 5.6-10.7% (CV, between-assay). Recovery was between 82 and 112%. Fasting plasma glucagon levels in diabetics, obese subjects, acromegalics and patients with Cushing's syndrome were greater than in normals (22.0 +/- 91 pg/ml; mean +/- SD). Very low glucagon levels after oral glucose suppression (15.2 +/- 3.1 pg/ml) in normals and greatly increased values after arginine in insulin-dependent diabetics (271.0 +/- 132.3 pg/ml) could be determined.

摘要

本文描述了一种使用针对胰腺胰高血糖素的特异性抗血清的胰高血糖素放射免疫测定法。胰高血糖素通过氯胺T技术进行放射性碘化，并在QAE-葡聚糖A 25上纯化，比活度达到225 μCi/μg。标准曲线的测量范围为12至500 pg/ml，灵敏度为17.5 pg/ml，精密度为6.3 - 14.9%（批内变异系数，CV）和5.6 - 10.7%（批间变异系数，CV）。回收率在82%至112%之间。糖尿病患者、肥胖受试者、肢端肥大症患者和库欣综合征患者的空腹血浆胰高血糖素水平高于正常人（22.0±91 pg/ml；平均值±标准差）。可以测定正常人口服葡萄糖后胰高血糖素水平非常低（15.2±3.1 pg/ml），而胰岛素依赖型糖尿病患者注射精氨酸后水平大幅升高（271.0±132.3 pg/ml）。