Alford F P, Bloodm S R, Nabarro J D
Diabetologia. 1977 Jan;13(1):1-6. doi: 10.1007/BF00996319.
Non-specific plasma effects may produce major errors in the estimation of true plasma pancreatic glucagon concentrations by radioimmunoassay. This has been circumvented by the production of glucagon-free plasma for each individual investigated, by means of glucagon antibody, coupled to sepharose beads. True fasting pancreatic glucagon levels (mean+/-SEM) in 18 healthy subjects (24+/-3 pg/ml) were significantly lower (p less than .005) than in 10 non-ketotic non-obese diabetics (38+/-3 pg/ml). It is suggested that, in the presence of decreased insulin-effect in the diabetic, this 55% glucagon elevation in diabetics may be of biological importance and contribute to the fasting hyperglycaemia.
非特异性血浆效应可能会在通过放射免疫测定法估算真实血浆胰高血糖素浓度时产生重大误差。通过使用与琼脂糖珠偶联的胰高血糖素抗体，为每个受调查个体制备无胰高血糖素血浆，这一问题得以解决。18名健康受试者的真实空腹胰高血糖素水平（均值±标准误）为（24±3 pg/ml），显著低于10名非酮症非肥胖糖尿病患者（38±3 pg/ml）（p<0.005）。有人提出，在糖尿病患者胰岛素效应降低的情况下，糖尿病患者胰高血糖素升高55%可能具有生物学意义，并导致空腹血糖升高。
