Hishiki S, Sakaguchi S, Kanno T
Clin Chim Acta. 1984 Jan 31;136(2-3):155-64. doi: 10.1016/0009-8981(84)90287-0.
Employing isoelectric focusing with an Ampholine column, the presence of three major pI isoenzymes (basic, neutral and acidic) of human ribonuclease (RNase) is demonstrated. The acidic pI isoenzyme was observed in pancreatic and hepatic cancer cells and in fetal pancreas and liver tissues, and was frequently detected in the sera of patients with pancreatic and hepatic cancer. This isoenzyme was not, however, detected in the sera of non-cancerous patients. In pancreatic cancer tissues, other pI isoenzymes, except for the acidic one, were markedly suppressed and the isoelectrophoretic patterns of the cancerous tissues closely resembled those obtained from fetal pancreatic tissue. These results suggest that the acidic pI isoenzyme could represent one of the carcinofetal proteins and that the detection of this pI isoenzyme in the patient's serum may be useful for the diagnosis of pancreatic and hepatic cancer.
利用两性电解质柱进行等电聚焦，证实了人核糖核酸酶（RNase）存在三种主要的等电点同工酶（碱性、中性和酸性）。酸性等电点同工酶在胰腺和肝癌细胞以及胎儿胰腺和肝脏组织中被观察到，并且在胰腺癌和肝癌患者的血清中经常被检测到。然而，在非癌症患者的血清中未检测到这种同工酶。在胰腺癌组织中，除酸性同工酶外，其他等电点同工酶明显受到抑制，癌组织的等电泳图谱与胎儿胰腺组织的图谱非常相似。这些结果表明，酸性等电点同工酶可能代表癌胚蛋白之一，在患者血清中检测这种等电点同工酶可能有助于胰腺癌和肝癌的诊断。
