Korhonen A, Hemminki K, Vainio H
Environ Res. 1984 Feb;33(1):54-61. doi: 10.1016/0013-9351(84)90008-2.
Nine peroxides were tested for embryotoxicity in 3-day chicken embryos using the air-chamber method. The potencies were expressed by the ED50 for the total embryotoxic effect of the chemicals, including deaths and malformations, up to Day 14 of the incubation. The range of the ED50's was from 0.13 to 2.7 mumoles per egg and the order of the potencies was as follows: cyclohexanoneperoxide greater than cumolhydroperoxide greater than ethylmethylketoneperoxide greater than dibenzoylperoxide greater than acetylacetoneperoxide greater than perbenzoic acid-tert-butylester greater than dicumylperoxide greater than dilauroylperoxide greater than hydrogen peroxide. All nine peroxides caused malformations at a moderate frequency. The maximum percentage of malformed embryos of the treated varied from the 16% of perbenzoic acid-tert-butylester to the 56% of dicumylperoxide. The high percentage caused by the latter could, however, result from slow diffusion of high lethal doses from the air chamber to the embryo.
使用气室法在3日龄鸡胚中对9种过氧化物进行了胚胎毒性测试。其效力通过化学物质对胚胎的总毒性作用（包括死亡和畸形）的半数有效剂量（ED50）来表示，直至孵化第14天。ED50的范围为每枚鸡蛋0.13至2.7微摩尔，效力顺序如下：环己酮过氧化物＞枯基过氧化氢＞乙基甲基酮过氧化物＞二苯甲酰过氧化物＞乙酰丙酮过氧化物＞叔丁基过苯甲酸酯＞二枯基过氧化物＞二月桂酰过氧化物＞过氧化氢。所有9种过氧化物都以中等频率导致畸形。处理组中畸形胚胎的最大百分比从叔丁基过苯甲酸酯的16%到二枯基过氧化物的56%不等。然而，后者导致的高百分比可能是由于高致死剂量从气室向胚胎的缓慢扩散所致。
