Vande Berg J S, Rudolph R, Woodward M
Am J Pathol. 1984 Feb;114(2):187-200.
Rat myofibroblasts from granulating wound biopsies (RGW) were successfully cultured and compared in terms of growth and morphology with fibroblasts from uninjured rat dermis (RD). Populations of early passage (P-3) RGW myofibroblasts grew significantly more slowly than RD fibroblasts. Logarithmic growth was nearly the same in late passage (P-30) populations of both cell types. Early passage RGW myofibroblasts were similar to those in vivo, as shown by well-defined microfilament bundles. RD fibroblasts contained less well defined microfilaments. Both late passage RGW myofibroblasts and RD fibroblasts displayed evidence of morphologic dedifferentiation. These data show that morphologic features of myofibroblasts and fibroblasts in vivo are maintained in vitro. Evidence is presented that cultured animal myofibroblasts maintain differentiation in early passage, whereas late passage cells suggest that these differences disappear with time.
成功培养了来自肉芽创面活检组织的大鼠肌成纤维细胞（RGW），并将其生长和形态与未受伤大鼠真皮成纤维细胞（RD）进行了比较。早期传代（P-3）的RGW肌成纤维细胞群体的生长明显比RD成纤维细胞缓慢。两种细胞类型的晚期传代（P-30）群体的对数生长期几乎相同。早期传代的RGW肌成纤维细胞与体内的相似，表现为微丝束清晰可辨。RD成纤维细胞所含的微丝则不那么清晰。晚期传代的RGW肌成纤维细胞和RD成纤维细胞均显示出形态去分化的迹象。这些数据表明，体内肌成纤维细胞和成纤维细胞的形态特征在体外得以维持。有证据表明，培养的动物肌成纤维细胞在早期传代时保持分化状态，而晚期传代细胞表明这些差异会随着时间消失。
