Matthews P N, Hermon-Taylor J, Grant A G
Br J Cancer. 1984 Feb;49(2):193-8. doi: 10.1038/bjc.1984.31.
The release of components from human kidney tumour xenografts (GYL) and human foetal kidney explants maintained in nude mice has been studied. The GYL tumour released antigens into the serum which could be detected by the generation of antibodies following cross-immunisation of closely related hairy litter mate (HLM) mice. The production of anti-GYL antibody was monitored by an I125 binding assay using viable GYL tumour cells. In 2/16 hairy litter mate mice, cell surface antibody binding by GYL cells was twice that found with 8 other human tumour cell lines (including 2 other kidney cancer cell lines). Absorption of these antisera with 10(7) GYL tumour cells completely abolished this response, where 50%, 38% and 25% of activity remained following absorption with; a normal kidney cell line, a homogenate of normal kidney and a mixed pool of human tumour cells. Six out of 8 GYL tumour bearing nude mice tested had elevated plasma levels of HCG. Absorption of the HLM antisera with an excess of commercial HCG abrogated I125 binding by only 15%, suggesting that antibody production was not directed primarily against ectopic HCG.
对裸鼠体内人肾肿瘤异种移植瘤（GYL）和人胎儿肾外植体中成分的释放进行了研究。GYL肿瘤向血清中释放抗原，通过对亲缘关系密切的有毛同窝仔鼠（HLM）进行交叉免疫后产生抗体来检测这些抗原。使用活的GYL肿瘤细胞通过I125结合试验监测抗GYL抗体的产生。在16只中有2只的有毛同窝仔鼠中，GYL细胞的细胞表面抗体结合是其他8种人肿瘤细胞系（包括另外2种肾癌细胞系）的两倍。用10⁷个GYL肿瘤细胞吸收这些抗血清可完全消除这种反应，而用正常肾细胞系、正常肾匀浆和人肿瘤细胞混合池吸收后，分别仍保留50%、38%和25%的活性。在测试的8只携带GYL肿瘤的裸鼠中，有6只的血浆HCG水平升高。用过量的商用HCG吸收HLM抗血清仅使I125结合减少15%，这表明抗体产生并非主要针对异位HCG。
