通过磁圆二色性对尿液和粪便中总卟啉进行快速半定量测量。

Rapid semiquantitative measurement of total porphyrins in urine and feces by magnetic circular dichroism.

作者信息

Ivanetich K M, Movsowitz C, Moore M R

出版信息

Clin Chem. 1984 Mar;30(3):391-4.

Abstract

Magnetic circular dichroism is a suitable technique for semiquantitative determination of total porphyrins in urine and feces, being rapid, reliable, and involving little sample preparation. The sensitivity of the assay suffices to distinguish normal from above-normal concentrations of total porphyrins in urine and feces. Results for urine compare well with those obtained by thin-layer chromatography of porphyrin esters (n = 42, r = 0.92) or ion-exchange column chromatography of porphyrin acids (n = 19, r = 0.80).

摘要

磁圆二色性是一种适用于半定量测定尿液和粪便中总卟啉的技术，该技术快速、可靠，且样品制备量少。该检测方法的灵敏度足以区分尿液和粪便中总卟啉浓度正常与高于正常的情况。尿液检测结果与通过卟啉酯薄层色谱法（n = 42，r = 0.92）或卟啉酸离子交换柱色谱法（n = 19，r = 0.80）获得的结果相当。