Carlson R E, Sivasothy R, Dolphin D, Bernstein M, Shivji A
Anal Biochem. 1984 Aug 1;140(2):360-5. doi: 10.1016/0003-2697(84)90178-7.
The routine clinical analysis of the porphyrin precursors of heme requires an internal standard to determine the efficiency of the analytical procedure used. 2-Vinyl-4-hydroxymethyldeuteroporphyrin IX has been prepared as an internal standard. Its application to porphyrin analysis has been demonstrated using high-performance liquid chromatographic resolution of the uro- to protoporphyrins in normal and porphyric urine.
