Elliott A Y, Bronson D L, Stein N, Fraley E E
Cancer Res. 1976 Feb;36(2 Pt 1):365-9.
Finely minced explants from 54 TCC2 of the human urinary tract were cultured in vitro in an attempt to establish cell lines. Cells with epithelial morphology grew out from 48 tumor explants, and long-term cell cultures were established from 10. Six of the cell cultures have been maintained for over 18 months with 50 to 70 transfers and, therefore, are considered permanent cell lines. The epithelial cells in the established cultures are small, exhibit rapid doubling time, and show multilayering. The cells were examined both microscopically and by cultivation techniques, and they were found to be free from contaminating microorganisms, including Mycoplasma. The established cultures grow rapidly in roller bottles and, therefore, can be produced in large quantities. These cells also remain viable after being stored for 3 years in liquid nitrogen.
对来自54例人泌尿道移行细胞癌2级（TCC2）的精细切碎的外植体进行体外培养，试图建立细胞系。48个肿瘤外植体长出了具有上皮形态的细胞，并从其中10个建立了长期细胞培养物。其中6个细胞培养物已维持了18个月以上，传代50至70次，因此被认为是永久细胞系。已建立的培养物中的上皮细胞体积小，倍增时间短，并呈现多层生长。对这些细胞进行了显微镜检查和培养技术检测，发现它们没有包括支原体在内的污染微生物。已建立的培养物在滚瓶中生长迅速，因此可以大量生产。这些细胞在液氮中储存3年后仍能存活。
