Dianzani F, Gullino P, Baron S
Infect Immun. 1978 Apr;20(1):55-7. doi: 10.1128/iai.20.1.55-57.1978.
Experiments were carried out to study the kinetics of local interferon production in the subcutaneous tissues of rats stimulated with Newcastle disease virus. Specifically, the interferon produced and released in the extracellular fluids was collected at various intervals of time in micropore chambers implanted into the subcutaneous tissue of rats. Interferon was detected at moderate titers 1 h after induction, and it was present at high titer at 2 h. The interferon levels remained remarkably high in the samples collected after 3, 5, and 24 h, and in some rats it was still detectable after 48 and 72 h. Since control experiments showed that it requires 2 to 3 h for interferon to penetrate the chambers, it may be concluded that high concentrations of interferon are present in the extracellular fluid within 1 h of induction. The evaluation of the kinetics of production and of the concentrations attained in the extracellular fluid suggests that in a solid tissue a cell infected by a potent interferon inducer may produce interferon early enough and in sufficient quantity to protect neighboring cells before the production of progeny virions.
进行了实验以研究新城疫病毒刺激大鼠皮下组织中局部干扰素产生的动力学。具体而言，在植入大鼠皮下组织的微孔室中，于不同时间间隔收集细胞外液中产生并释放的干扰素。诱导后1小时可检测到中等滴度的干扰素，2小时时出现高滴度。在3、5和24小时后收集的样本中，干扰素水平仍显著较高，在一些大鼠中，48和72小时后仍可检测到。由于对照实验表明干扰素穿透微孔室需要2至3小时，因此可以得出结论，诱导后1小时内细胞外液中存在高浓度的干扰素。对细胞外液中干扰素产生动力学和达到的浓度的评估表明，在实体组织中，被强效干扰素诱导剂感染的细胞可能足够早且足够量地产生干扰素，以在子代病毒粒子产生之前保护邻近细胞。
