Saris S C, Bigner S H, Bigner D D
J Neurosurg. 1984 Mar;60(3):582-8. doi: 10.3171/jns.1984.60.3.0582.
A model was developed for in vivo study of the human glioma-derived D-54 MG cell line in the brains of immunosuppressed Fischer 344 rats. The rats were injected with horse anti-rat thymocyte serum before and after intracerebral inoculation with 5 or 10 microliters of a D-54 MG tumor cell suspension. Reproducible mortality distributions were obtained, with deaths occurring 18 to 34 days after intracerebral inoculation. Tumors grew as well circumscribed intracerebral masses with sheets of anaplastic cells, areas of necrosis bordered by concentrated nuclei, and minimal lymphocytic infiltration. Cytogenetic analysis revealed the same general chromosome distribution and markers in the heterotransplanted glioma cells as in the cultured line. Blood-brain barrier disruption was demonstrated by intracerebral tumor staining after intravenous injection of Evans blue dye. The in vivo growth of D-54 MG in immunosuppressed rats provides a reliable experimental model for the study of chemotherapy, immunodiagnosis, and immunotherapy of a human glioma-derived tumor in an animal sufficiently large to evaluate intracarotid or intratumoral injection of therapeutic agents.
建立了一种用于在免疫抑制的Fischer 344大鼠脑内对人胶质瘤来源的D-54 MG细胞系进行体内研究的模型。在脑内接种5或10微升D-54 MG肿瘤细胞悬液之前和之后，给大鼠注射马抗大鼠胸腺细胞血清。获得了可重复的死亡率分布，脑内接种后18至34天出现死亡。肿瘤生长为界限清楚的脑内肿块，有间变细胞片、由浓缩细胞核界定的坏死区域和极少的淋巴细胞浸润。细胞遗传学分析显示，异种移植的胶质瘤细胞中的一般染色体分布和标记与培养细胞系中的相同。静脉注射伊文思蓝染料后，脑内肿瘤染色证明了血脑屏障的破坏。D-54 MG在免疫抑制大鼠体内的生长为研究人胶质瘤来源肿瘤的化疗、免疫诊断和免疫治疗提供了一个可靠的实验模型，该动物足够大，可评估颈内或瘤内注射治疗剂。
