Bulte J W, Go K G, Zuiderveen F, The T H, de Leij L
Department of Clinical Immunology, University of Groningen, The Netherlands.
J Neurooncol. 1993 Apr;16(1):11-8. doi: 10.1007/BF01324829.
In the WAG/Rij nude rat, subcutaneous (s.c.) and intracerebral (i.c.) xenografts of the human SCLC cell line GLC-28 were evaluated for their growth behavior, in vivo monoclonal antibody binding and presence of tumor infiltrating lymphocytes. For the i.c. xenografts, two models of cerebral tumor growth were studied, one in the cerebral cortex and one in the lateral ventricle of the brain. In the s.c. and both i.c. xenografts models, in vivo localization of anti-carcinoma moab MOC-31 occurred within 4 hours after i.p. injection, with a maximal binding at 24 h after injection. A pronounced tumor infiltration of predominantly NK cells was observed for s.c. and intraventricular xenografts, but not for the GLC-28 tumors xenografted in the cerebral cortex. The presented nude rat/GLC-28 xenograft models may be used for the in vivo testing of experimental imaging techniques or alternative treatment strategies relevant to brain metastases of human SCLC.
在WAG/Rij裸鼠中，对人小细胞肺癌细胞系GLC-28的皮下（s.c.）和脑内（i.c.）异种移植瘤的生长行为、体内单克隆抗体结合情况以及肿瘤浸润淋巴细胞的存在进行了评估。对于脑内异种移植瘤，研究了两种脑肿瘤生长模型，一种在大脑皮层，另一种在脑侧脑室。在皮下和两种脑内异种移植瘤模型中，腹腔注射后4小时内抗癌单克隆抗体MOC-31在体内发生定位，注射后24小时结合达到最大值。皮下和脑室内异种移植瘤观察到主要为NK细胞的明显肿瘤浸润，但大脑皮层异种移植的GLC-28肿瘤未观察到。所呈现的裸鼠/GLC-28异种移植瘤模型可用于体内测试与人类小细胞肺癌脑转移相关的实验成像技术或替代治疗策略。
