Whittaker J L, Byrd P J, Grand R J, Gallimore P H
Mol Cell Biol. 1984 Jan;4(1):110-6. doi: 10.1128/mcb.4.1.110-116.1984.
Four transformed cell lines were established from cultures of human embryo kidney (HEK) cells microinjected or transfected with cloned adenovirus 12 (Ad12) EcoRI-C DNA (0 through 16.5 map units of the left-hand end of the viral genome). Each cell line showed a different growth pattern. Southern blotting demonstrated that all of the cell lines contained Ad12-specific DNA sequences, but in the microinjected isolates these were at a much lower copy number than in the transfected isolate. Two cell lines (Ad12 HEK 1 and 3) appeared to contain tandemly repeated Ad12 EcoRI-C DNA fragments. Immunoprecipitation and Western blotting confirmed that Ad12 early region 1 (E1) proteins were being expressed by all four of the transformed cell lines, but indicated that E1A polypeptide expression was considerably less than E1B polypeptide expression. All of the Ad12-transformed HEK cell lines were tumorigenic when inoculated intracranially into athymic nude mice.
从经显微注射或转染克隆腺病毒12（Ad12）EcoRI - C DNA（病毒基因组左端0至16.5个图谱单位）的人胚肾（HEK）细胞培养物中建立了四种转化细胞系。每个细胞系都呈现出不同的生长模式。Southern印迹法表明，所有细胞系都含有Ad12特异性DNA序列，但在显微注射分离株中，这些序列的拷贝数远低于转染分离株。两个细胞系（Ad12 HEK 1和3）似乎含有串联重复的Ad12 EcoRI - C DNA片段。免疫沉淀和Western印迹证实，所有四个转化细胞系都在表达Ad12早期区域1（E1）蛋白，但表明E1A多肽的表达远低于E1B多肽的表达。当将所有Ad12转化的HEK细胞系颅内接种到无胸腺裸鼠中时，它们都具有致瘤性。
