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用于核酸偶联和杂交的重氮化芳胺纤维素纸。

Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids.

作者信息

Seed B

出版信息

Nucleic Acids Res. 1982 Mar 11;10(5):1799-810. doi: 10.1093/nar/10.5.1799.

DOI:10.1093/nar/10.5.1799
PMID:7071022
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320567/
Abstract

We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by blot transfer and hybridization.

摘要

我们描述了一类芳胺取代纸的合成方法，这类纸可进行重氮化并与核酸偶联。合成方法简单，使用的起始原料易于获得。本文报道了核酸结合活性的部分表征，以及活化纸在通过印迹转移和杂交检测电泳分离的RNA中的应用演示。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/188d/320567/dbf176b4cd29/nar00374-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/188d/320567/dbf176b4cd29/nar00374-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/188d/320567/dbf176b4cd29/nar00374-0380-a.jpg

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1
Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids.用于核酸偶联和杂交的重氮化芳胺纤维素纸。
Nucleic Acids Res. 1982 Mar 11;10(5):1799-810. doi: 10.1093/nar/10.5.1799.
2
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3
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本文引用的文献

1
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.变性RNA与转移至硝酸纤维素膜上的小DNA片段的杂交。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5. doi: 10.1073/pnas.77.9.5201.
2
The chemistry and use of cellulose derivatives for the study of biological systems.用于生物系统研究的纤维素衍生物的化学性质及应用
Immunochemistry. 1965 Dec;2(4):293-322. doi: 10.1016/0019-2791(65)90031-5.
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Preparation of adsorbents for biospecific affinity chromatography. Attachment of group-containing ligands to insoluble polymers by means of bifuctional oxiranes.
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Isolation and characterization of a gene for a major light-harvesting polypeptide from Cyanophora paradoxa.从蓝藻 Cyanophora paradoxa 中分离和鉴定一个主要捕光多肽的基因。
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4100-4. doi: 10.1073/pnas.81.13.4100.
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Conserved sequence elements in the 5' region of the Ultrabithorax transcription unit.Ubx 转录单元 5' 区的保守序列元件。
EMBO J. 1987 May;6(5):1393-401. doi: 10.1002/j.1460-2075.1987.tb02380.x.
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Cytochrome oxidase subunit II gene in mitochondria of Oenothera has no intron.线粒体细胞色素氧化酶亚基 II 基因在月见草中没有内含子。
EMBO J. 1983;2(12):2173-8. doi: 10.1002/j.1460-2075.1983.tb01719.x.
7
Role of cucumber mosaic virus and its satellite RNA in the etiology of tomato fruit necrosis in Italy.黄瓜花叶病毒及其卫星RNA在意大利番茄果实坏死病因学中的作用。
Arch Virol. 1993;131(3-4):321-33. doi: 10.1007/BF01378635.
8
Protein D1 preferentially binds A + T-rich DNA in vitro and is a component of Drosophila melanogaster nucleosomes containing A + T-rich satellite DNA.蛋白质D1在体外优先结合富含A+T的DNA，并且是含有富含A+T卫星DNA的黑腹果蝇核小体的一个组成部分。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7152-6. doi: 10.1073/pnas.79.23.7152.
9
A new blotting medium for the simple isolation and identification of highly resolved messenger RNA.一种用于简单分离和鉴定高分辨率信使核糖核酸的新型印迹介质。
Nucleic Acids Res. 1984 Feb 10;12(3):1349-59. doi: 10.1093/nar/12.3.1349.
10
Common and distinct regions of defective-interfering RNAs of Sindbis virus.辛德毕斯病毒缺陷干扰RNA的共同和独特区域
J Virol. 1984 Mar;49(3):865-72. doi: 10.1128/JVI.49.3.865-872.1984.
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5
Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.从甲醛琼脂糖凝胶转移至重氮苄氧基甲基纸上的前胶原信使核糖核酸的鉴定
Nucleic Acids Res. 1979 Aug 10;6(11):3559-67. doi: 10.1093/nar/6.11.3559.
6
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.通过转移至重氮苄氧基甲基纸并与DNA探针杂交来检测琼脂糖凝胶中特定RNA的方法。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4. doi: 10.1073/pnas.74.12.5350.
7
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.通过使用硫酸葡聚糖将大的DNA片段从琼脂糖凝胶高效转移至重氮苄氧基甲基纸并进行快速杂交。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7. doi: 10.1073/pnas.76.8.3683.
8
Nucleic acid hybridization using DNA covalently coupled to cellulose.使用共价偶联到纤维素上的DNA进行核酸杂交。
Cell. 1975 Jul;5(3):301-10. doi: 10.1016/0092-8674(75)90105-1.
9
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.通过凝胶分级分离并转移至重氮苄氧基甲基纸上检测特定RNA或DNA特定片段。
Methods Enzymol. 1979;68:220-42. doi: 10.1016/0076-6879(79)68017-5.
10
Isolation of specific RNA's using DNA covalently linked to diazobenzyloxymethyl cellulose or paper.使用与重氮苄氧基甲基纤维素或滤纸共价连接的DNA分离特定RNA。
Methods Enzymol. 1979;68:206-20. doi: 10.1016/0076-6879(79)68016-3.