Amdur B H, Szabo E I, Socransky S S
J Bacteriol. 1978 Jul;135(1):161-3. doi: 10.1128/jb.135.1.161-163.1978.
The presence of the isoprenoid squalene, synthesized de novo, was demonstrated in 64 out of 73 strains of gram-positive bacteria by thin-layer chromatography. This observation was confirmed by gas-liquid chromatography, chemical reactivity, incorporation of radiolabeled precursor, and by gas chromatography mass spectroscopy of thin-layer chromatography-recovered material.
通过薄层色谱法在73株革兰氏阳性菌中的64株中证实了从头合成的类异戊二烯角鲨烯的存在。这一观察结果通过气液色谱法、化学反应性、放射性标记前体的掺入以及薄层色谱回收材料的气相色谱-质谱法得到了证实。