肺炎链球菌噬菌体Cp-1 DNA末端的核苷酸序列。

Nucleotide sequence at the termini of the DNA of Streptococcus pneumoniae phage Cp-1.

作者信息

Escarmís C, Gómez A, García E, Ronda C, López R, Salas M

出版信息

Virology. 1984 Feb;133(1):166-71. doi: 10.1016/0042-6822(84)90435-5.

Abstract

The 5' ends of Cp-1 DNA, which have a covalently linked terminal protein, can be partially unblocked by treatment with 1 M NaOH (E. Garcia, A. Gomez, C. Ronda, C. Escarmis, and R. Lopez (1983) Virology 128, 92-104) and labeled with polynucleotide kinase and [gamma-32P]ATP. The sequence of the first 444 and 520 nucleotides at the termini of Cp-1 DNA has been determined. A 236-nucleotide-long inverted terminal repeat was found and, in addition, the 116 nucleotides following the repeat show 93% homology. The first 352 nucleotides at both ends have an adenine plus thymine content of 75%. More than 50% of the nucleotides of the sequenced regions are involved in repeats of a minimum of 8 nucleotides. Three promoter-like sequences were also found at each end of Cp-1 DNA.

摘要

Cp-1 DNA的5'端带有共价连接的末端蛋白，用1 M NaOH处理可使其部分解封闭（E. 加西亚、A. 戈麦斯、C. 龙达、C. 埃斯卡米斯和R. 洛佩斯（1983年），《病毒学》128卷，第92 - 104页），然后用多核苷酸激酶和[γ-32P]ATP进行标记。已确定了Cp-1 DNA末端最初的444和520个核苷酸的序列。发现了一个236个核苷酸长的反向末端重复序列，此外，该重复序列之后的116个核苷酸显示出93%的同源性。两端最初的352个核苷酸中腺嘌呤加胸腺嘧啶的含量为75%。测序区域中超过50%的核苷酸参与了至少8个核苷酸的重复。在Cp-1 DNA的两端还发现了三个类似启动子的序列。