• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

肺炎链球菌噬菌体DNA的限制性切割图谱，这些噬菌体的5'端含有共价结合的蛋白质。

Restriction cleavage maps of the DNAs of Streptococcus pneumoniae bacteriophages containing protein covalently bound to their 5' ends.

作者信息

López R, Ronda C, García P, Escarmís C, García E

出版信息

Mol Gen Genet. 1984;197(1):67-74. doi: 10.1007/BF00327924.

DOI:10.1007/BF00327924
PMID:6096673
Abstract

Several pneumococcal bacteriophages showing a morphology similar to that previously described for Cp-1 (Ronda et al. 1981) have been isolated and purified from throat samples taken from healthy children. Three of these phages (Cp-5, Cp-7 and Cp-9) have been studied in detail and compared to Cp-1. The four phages differed in several respects, e.g. size, structural polypeptides, restriction enzyme cleavage patterns, etc. The DNA of Cp-5, Cp-7 and Cp-9 showed protease-sensitive transfecting activity. This, together with the results obtained by electrophoretic analyses as well as by isotopic labelling of these DNAs with [gamma-32P]ATP and polynucleotide kinase indicated that all these new phages have a protein covalently linked to the 5' ends of their DNAs as in the case of Cp-1 (García et al. 1983). Restriction enzyme cleavage maps of Cp-1, Cp-5, Cp-7 and Cp-9 have been constructed.

摘要

已从健康儿童的咽喉样本中分离并纯化出几种肺炎球菌噬菌体，其形态与先前描述的Cp-1噬菌体相似（Ronda等人，1981年）。其中三种噬菌体（Cp-5、Cp-7和Cp-9）已进行了详细研究，并与Cp-1进行了比较。这四种噬菌体在几个方面存在差异，例如大小、结构多肽、限制性内切酶切割图谱等。Cp-5、Cp-7和Cp-9的DNA显示出蛋白酶敏感的转染活性。这与电泳分析以及用[γ-32P]ATP和多核苷酸激酶对这些DNA进行同位素标记所获得的结果一起表明，所有这些新噬菌体都有一个与DNA 5'末端共价连接的蛋白质，就像Cp-1噬菌体那样（García等人，1983年）。已构建了Cp-1、Cp-5、Cp-7和Cp-9的限制性内切酶切割图谱。

相似文献

1
Restriction cleavage maps of the DNAs of Streptococcus pneumoniae bacteriophages containing protein covalently bound to their 5' ends.肺炎链球菌噬菌体DNA的限制性切割图谱，这些噬菌体的5'端含有共价结合的蛋白质。
Mol Gen Genet. 1984;197(1):67-74. doi: 10.1007/BF00327924.
2
Nucleotide sequence at the termini of the DNA of Streptococcus pneumoniae phage Cp-1.肺炎链球菌噬菌体Cp-1 DNA末端的核苷酸序列。
Virology. 1984 Feb;133(1):166-71. doi: 10.1016/0042-6822(84)90435-5.
3
Bacteriophages of Streptococcus pneumoniae: a molecular approach.肺炎链球菌的噬菌体：一种分子方法。
Microb Drug Resist. 1997 Summer;3(2):165-76. doi: 10.1089/mdr.1997.3.165.
4
Temperate bacteriophages of Streptococcus pneumoniae that contain protein covalently linked to the 5' ends of their DNA.肺炎链球菌的温和噬菌体，其DNA的5'端含有共价连接的蛋白质。
J Virol. 1990 Oct;64(10):5149-55. doi: 10.1128/JVI.64.10.5149-5155.1990.
5
Pneumococcal bacteriophage Cp-1 contains a protein bound to the 5' termini of its DNA.肺炎球菌噬菌体Cp-1含有一种与DNA 5'末端结合的蛋白质。
Virology. 1983 Jul 15;128(1):92-104. doi: 10.1016/0042-6822(83)90321-5.
6
Protease-sensitive transfection of Streptococcus pneumoniae with bacteriophage Cp-1 DNA.用噬菌体Cp-1 DNA对肺炎链球菌进行蛋白酶敏感转染。
J Virol. 1983 Dec;48(3):721-30. doi: 10.1128/JVI.48.3.721-730.1983.
7
Formation of a covalent complex between the terminal protein of pneumococcal bacteriophage Cp-1 and 5'-dAMP.肺炎球菌噬菌体Cp-1的末端蛋白与5'-脱氧腺苷一磷酸之间共价复合物的形成。
J Virol. 1986 Apr;58(1):31-5. doi: 10.1128/JVI.58.1.31-35.1986.
8
Inverted terminal repeats and terminal proteins of the genomes of pneumococcal phages.
Gene. 1985;36(3):341-8. doi: 10.1016/0378-1119(85)90189-1.
9
[Wide distribution of transposable phages in natural Pseudomonas aeruginosa populations].[转座噬菌体在天然铜绿假单胞菌群体中的广泛分布]
Genetika. 1984 Oct;20(10):1612-9.
10
Infection of Streptococcus oralis NCTC 11427 by pneumococcal phages.
FEMS Microbiol Lett. 1989 Nov;53(1-2):187-92. doi: 10.1016/0378-1097(89)90389-3.

引用本文的文献

1
Terminal protein association and sequence homology in linear mitochondrial plasmid-like DNAs of sorghum and maize.线性线粒体类似质粒 DNA 中高粱和玉米末端蛋白的关联和序列同源性。
Plant Cell Rep. 1987 Apr;6(2):127-30. doi: 10.1007/BF00276669.
2
Molecular cloning and physical characterization of a Brassica linear mitochondrial plasmid.一种甘蓝型油菜线性线粒体质粒的分子克隆及物理特性分析
Mol Gen Genet. 1987 Sep;209(2):227-33. doi: 10.1007/BF00329647.
3
Functional analysis of the two-gene lysis system of the pneumococcal phage Cp-1 in homologous and heterologous host cells.

本文引用的文献

1
Nucleotide sequence of the S-2 mitochondrial DNA from the S cytoplasm of maize.玉米 S 细胞质中 S-2 线粒体 DNA 的核苷酸序列。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4055-9. doi: 10.1073/pnas.80.13.4055.
2
S1 and S2, the linear mitochondrial DNAs present in a male sterile line of maize, possess terminally attached proteins.S1和S2是玉米雄性不育系中存在的线性线粒体DNA，它们带有末端连接蛋白。
Nucleic Acids Res. 1982 Dec 20;10(24):8181-90. doi: 10.1093/nar/10.24.8181.
3
Eukaryotic DNA replication: viral and plasmid model systems.
肺炎球菌噬菌体Cp-1双基因裂解系统在同源和异源宿主细胞中的功能分析。
J Bacteriol. 1998 Jan;180(2):210-7. doi: 10.1128/JB.180.2.210-217.1998.
4
Analysis of the complete nucleotide sequence and functional organization of the genome of Streptococcus pneumoniae bacteriophage Cp-1.肺炎链球菌噬菌体Cp-1基因组的完整核苷酸序列及功能组织分析。
J Virol. 1996 Jun;70(6):3678-87. doi: 10.1128/JVI.70.6.3678-3687.1996.
5
Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage phi 29.
Nucleic Acids Res. 1985 Nov 11;13(21):7715-28. doi: 10.1093/nar/13.21.7715.
6
Nucleotide sequence of Bacillus phage Nf terminal protein gene.芽孢杆菌噬菌体Nf末端蛋白基因的核苷酸序列
Nucleic Acids Res. 1987 Jul 10;15(13):5251-9. doi: 10.1093/nar/15.13.5251.
7
Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin.肺炎球菌噬菌体Cp-1溶素的克隆、纯化及生化特性分析
J Virol. 1987 Aug;61(8):2573-80. doi: 10.1128/JVI.61.8.2573-2580.1987.
8
Characterization of genetic transformation in Streptococcus oralis NCTC 11427: expression of the pneumococcal amidase in S. oralis using a new shuttle vector.口腔链球菌NCTC 11427基因转化的特性：使用新型穿梭载体在口腔链球菌中表达肺炎球菌酰胺酶
Mol Gen Genet. 1988 Dec;215(1):53-7. doi: 10.1007/BF00331302.
9
Formation of a covalent complex between the terminal protein of pneumococcal bacteriophage Cp-1 and 5'-dAMP.肺炎球菌噬菌体Cp-1的末端蛋白与5'-脱氧腺苷一磷酸之间共价复合物的形成。
J Virol. 1986 Apr;58(1):31-5. doi: 10.1128/JVI.58.1.31-35.1986.
10
Isolation, characterization and physiological properties of an autolytic-deficient mutant of Streptococcus pneumoniae.
Mol Gen Genet. 1986 Aug;204(2):237-42. doi: 10.1007/BF00425504.
真核生物DNA复制：病毒和质粒模型系统
Annu Rev Biochem. 1982;51:901-34. doi: 10.1146/annurev.bi.51.070182.004345.
4
Initiation of phage phi 29 DNA replication in vitro: formation of a covalent complex between the terminal protein, p3, and 5'-dAMP.噬菌体φ29 DNA体外复制的起始：末端蛋白p3与5'-dAMP之间共价复合物的形成。
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5522-6. doi: 10.1073/pnas.79.18.5522.
5
Nucleotide sequence at the termini of the DNA of Streptococcus pneumoniae phage Cp-1.肺炎链球菌噬菌体Cp-1 DNA末端的核苷酸序列。
Virology. 1984 Feb;133(1):166-71. doi: 10.1016/0042-6822(84)90435-5.
6
Identification of a protein bound to the termini of bacteriophage PRD1 DNA.与噬菌体PRD1 DNA末端结合的一种蛋白质的鉴定。
J Virol. 1983 Aug;47(2):311-6. doi: 10.1128/JVI.47.2.311-316.1983.
7
A new mechanism for the initiation of replication of phi 29 and adenovirus DNA: priming by the terminal protein.
Curr Top Microbiol Immunol. 1984;109:89-106. doi: 10.1007/978-3-642-69460-8_4.
8
Protease-sensitive transfection of Streptococcus pneumoniae with bacteriophage Cp-1 DNA.用噬菌体Cp-1 DNA对肺炎链球菌进行蛋白酶敏感转染。
J Virol. 1983 Dec;48(3):721-30. doi: 10.1128/JVI.48.3.721-730.1983.
9
A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.一种来自罗氏链霉菌的线性DNA质粒，具有614个碱基对的反向末端重复序列。
EMBO J. 1984 Apr;3(4):761-6. doi: 10.1002/j.1460-2075.1984.tb01881.x.
10
Template requirements for initiation of phage phi 29 DNA replication in vitro.体外启动噬菌体φ29 DNA复制的模板要求。
Proc Natl Acad Sci U S A. 1984 Jan;81(1):80-4. doi: 10.1073/pnas.81.1.80.