Harel L, Jullien M, de Monti M
J Cell Physiol. 1978 Sep;96(3):327-32. doi: 10.1002/jcp.1040960308.
In 3T3 Swiss mouse fibroblasts, incorporation of phosphate into cells and phosphorylation of small organic compounds were increased by shaking dense cultures. This response was not obtained with SV40 transformed Swiss 3T3 cells (SV-3T3). It appeared likely that these results could be accounted for by an inhibitor released from 3T3 cells but not from SV-3T3 cells. Our new method of co-incubation of sparse and dense cultures allowed us to demonstrate inhibition of growth and phosphate metabolism in sparse 3T3 cultures which were shaken in the presence of dense cultures. The inhibition was much less when the cultures were co-cultivated but not shaken. The inhibition of phosphate incorporation in acid-soluble and acid-insoluble fractions of sparse cultures was observed as early as 20 minutes of co-incubation in the presence of dense cultures, so this inhibition is not the result of depletion of growth factors in the medium. Our experiments suggest that an inhibitor(s) was released from dense cultures of 3T3 cells.
在3T3瑞士小鼠成纤维细胞中，通过振荡高密度培养物，细胞对磷酸盐的摄取以及小分子有机化合物的磷酸化作用增强。而SV40转化的瑞士3T3细胞（SV - 3T3）未出现这种反应。这些结果似乎可以用3T3细胞释放的一种抑制剂来解释，而SV - 3T3细胞不释放这种抑制剂。我们新的稀疏培养物与高密度培养物共同孵育的方法，使我们能够证明在与高密度培养物一起振荡的稀疏3T3培养物中，生长和磷酸盐代谢受到抑制。当培养物共同培养但不振荡时，抑制作用要小得多。在与高密度培养物共同孵育时，早在20分钟就观察到稀疏培养物的酸溶性和酸不溶性部分中磷酸盐摄取受到抑制，所以这种抑制不是培养基中生长因子耗尽的结果。我们的实验表明，3T3细胞的高密度培养物释放了一种抑制剂。
