van der Bosch J
J Cell Physiol. 1980 Aug;104(2):127-36. doi: 10.1002/jcp.1041040202.
An aging process has been detected in stationary 3T3 cell cultures, especially in the presence of plasma-derived serum (PDS) from adult bulls. It leads to irreversible conversion of an increasing percentage of initially responsive cells of a stationary population into cells unresponsive to growth stimulation by newborn calf serum (NBCS) or reseeding at low cell density in the presence of NBCS. These unresponsive cells are viable in the sense that, following trypsinization, they reattach and spread on a new culture plate and can be maintained for many days. The conversion process is accelerated by increasing PDS concentration. It is antagonized by NBCS. It is accompanied by enhancement of growth-inhibiting interactions exerted by stationary 3T3 cell populations on SV 40-3T3 cells.
在静止的3T3细胞培养物中检测到了衰老过程，尤其是在存在成年公牛血浆来源血清（PDS）的情况下。这导致静止群体中越来越多最初有反应的细胞不可逆地转化为对新生牛血清（NBCS）的生长刺激无反应的细胞，或者在有NBCS的情况下以低细胞密度重新接种时无反应的细胞。这些无反应的细胞在胰蛋白酶消化后能够重新附着并在新的培养板上扩散，并且可以维持许多天，从这个意义上来说它们是有活力的。转化过程会随着PDS浓度的增加而加速。它受到NBCS的拮抗。它伴随着静止的3T3细胞群体对SV 40 - 3T3细胞施加的生长抑制相互作用的增强。
