Gamble H R, Graham C E
Am J Vet Res. 1984 Jan;45(1):67-74.
Monoclonal antibodies generated from spleen cells of Balb/cJ mice infected with Trichinella spiralis reacted with antigenic determinants present in some other common swine parasites, especially Trichuris suis. An antigenic determinant unique to T spiralis was recognized by one immunoglobulin M monoclonal antibody (7C2C5); this determinant was found on larval T spiralis proteins with molecular weights of 53,000, 49,000, and 45,000. These proteins were components of in vitro-derived worm excretory-secretory products. Immunoperoxidase staining techniques and reactions of the monoclonal antibody with living parasites indicated that the source of the antigen was the stichocyte. Two of the proteins (53,000 and 49,000 molecular weight) possessing this determinant were isolated by monoclonal antibody-affinity chromatography, using hybridoma 7C2C5. When used in a serologic enzyme-linked immunosorbent assay, the affinity-isolated antigen was able to completely eliminate false-positive reactions with serum from healthy swine and to detect all pigs infected with T spiralis.
从感染旋毛虫的Balb/cJ小鼠脾脏细胞产生的单克隆抗体与一些其他常见猪寄生虫（特别是猪鞭虫）中存在的抗原决定簇发生反应。一种免疫球蛋白M单克隆抗体（7C2C5）识别出旋毛虫特有的一个抗原决定簇；该决定簇存在于分子量为53000、49000和45000的旋毛虫幼虫蛋白上。这些蛋白是体外培养的虫体排泄-分泌产物的组成成分。免疫过氧化物酶染色技术以及单克隆抗体与活寄生虫的反应表明，抗原来源是杆状体细胞。使用杂交瘤7C2C5通过单克隆抗体亲和层析分离出了两种具有该决定簇的蛋白（分子量分别为53000和49000）。当用于血清学酶联免疫吸附试验时，亲和分离的抗原能够完全消除与健康猪血清的假阳性反应，并检测出所有感染旋毛虫的猪。
