Ataullakhanov F I, Vitvitskiĭ V M, Zhabotinskiĭ A M, Pichugin A V, Pomazanov V V
Biokhimiia. 1984 Jan;49(1):104-10.
The ATP content in human erythrocytes depleted without glucose falls down to half of the initial value within 2-3 hours and reaches practically zero within more than 10 hours. The ADP content increases 2-3-fold during the 1st hour after depletion and then slowly decreases. The AMP content increases 10-fold during several hours, but the rate of this process constantly decreases. The adenylate pool decreases at a constant rate ranging from 0.13 to 0.25 mmol/l cell. h; this is accompanied by accumulation of IMP. Addition of glucose to depleted erythrocytes results in partial recovery of the ATP level within 1-2 hours. The sooner glucose addition after the depletion, the greater the recovery. Simultaneously the ADP and AMP levels drastically decrease to new constant values. The decline of the adenylate pool ceases and the rate of IMP accumulation increases. Normally, the [ATP]/adenylate pool ratio lies within the small interval 0.85-0.94 irrespective of significant individual differences in the absolute values of [ATP]. This ratio is decreased during depletion and restored to the initial value after glucose addition. The mass-action ratio of the adenylate kinase reaction changes greatly during depletion and restoration of erythrocyte ATP.
在无葡萄糖条件下耗尽的人红细胞中的ATP含量在2 - 3小时内降至初始值的一半，并在10多个小时内几乎降至零。ADP含量在耗尽后的第1小时内增加2 - 3倍，然后缓慢下降。AMP含量在数小时内增加10倍，但该过程的速率不断降低。腺苷酸库以0.13至0.25 mmol/l细胞·小时的恒定速率下降；同时伴随着IMP的积累。向耗尽的红细胞中添加葡萄糖会导致ATP水平在1 - 2小时内部分恢复。耗尽后添加葡萄糖越早，恢复程度越大。同时，ADP和AMP水平急剧下降至新的恒定值。腺苷酸库的下降停止，IMP积累速率增加。正常情况下，[ATP]/腺苷酸库比值处于0.85 - 0.94的小范围内，与[ATP]绝对值的显著个体差异无关。该比值在耗尽期间降低，并在添加葡萄糖后恢复到初始值。在红细胞ATP耗尽和恢复过程中，腺苷酸激酶反应的质量作用比变化很大。
