Rocchiccioli F, Leroux J P, Cartier P H
Biomed Mass Spectrom. 1984 Jan;11(1):24-8. doi: 10.1002/bms.1200110105.
Quantification of 2-ketoglutaric acid in plasma and cerebrospinal fluid as its O-trimethylsilyl++-quinoxalinol derivative by gas chromatography chemical ionization mass spectrometry is described with benzoylformic acid as internal standard. This technique, with ammonia as reactant gas, only detects the protonated molecular ions. The recovery of 2-ketoglutarate from perchloric-deproteinized plasma is 99.7 +/- 1.2%. The normal value of 2-ketoglutarate in children is 8.6 +/- 2.6 mumol l-1 (mean +/- standard deviation) in plasma (n = 25) and 4.8 +/- 1.4 mumol l-1 in cerebrospinal fluid (n = 20). The plasma level of 2-ketoglutarate is correlated with urea concentration (r = 0.96; p less than 0.001) in healthy subjects and in patients with chronic renal insufficiency. Increased values are found in one case of pyruvate carboxylase deficiency, and inconstantly in diabetes; physiological variations are described during fasting and after an oral glucose load.
本文描述了以苯甲酰甲酸为内标，通过气相色谱化学电离质谱法对血浆和脑脊液中2-酮戊二酸作为其O-三甲基硅基++-喹喔啉醇衍生物进行定量分析的方法。该技术以氨作为反应气，仅检测质子化分子离子。从高氯酸脱蛋白血浆中回收2-酮戊二酸的回收率为99.7±1.2%。儿童血浆中2-酮戊二酸的正常值为8.6±2.6μmol l-1（平均值±标准差，n = 25），脑脊液中为4.8±1.4μmol l-1（n = 20）。在健康受试者和慢性肾功能不全患者中，血浆中2-酮戊二酸水平与尿素浓度相关（r = 0.96；p＜0.001）。在1例丙酮酸羧化酶缺乏症患者中发现值升高，在糖尿病患者中则不恒定；描述了禁食期间和口服葡萄糖负荷后的生理变化。
