Brainard G C, Richardson B A, King T S, Reiter R J
Brain Res. 1984 Mar 5;294(2):333-9. doi: 10.1016/0006-8993(84)91045-x.
The purpose of this study was to test the capacity of different visible wavelengths of light to suppress nocturnal levels of pineal melatonin in hamsters. It was found that the visible wavelengths vary in their ability to perturb pineal melatonin. During the period of peak pineal melatonin production, animals were exposed to fluorescent light sources having half-peak bandwidths of 339-371 nm (near-ultraviolet), 435-500 nm (blue), 510-550 nm (green), 558-636 nm (yellow) and 653-668 nm (red). In each experiment, animals were exposed to equal irradiances of each light source. The different irradiances used were 0.928, 0.200, 0.186, 0.074 and 0.019 microW/cm2. The resultant data demonstrated that blue fluorescent light was the most efficient in suppressing pineal melatonin. Green fluorescent light was found to be the next most efficient light for inhibiting pineal melatonin followed by yellow fluorescent light. Near-ultraviolet and red light were the least capable of suppressing pineal melatonin. These observations suggest that the retinal photopigment responsible for mediating the pineal gland's response to light in the hamster may be either rhodopsin or another blue-sensitive chromophore.
本研究的目的是测试不同可见波长的光抑制仓鼠松果体褪黑素夜间水平的能力。结果发现，可见波长在干扰松果体褪黑素的能力方面存在差异。在松果体褪黑素分泌高峰期，将动物暴露于半峰带宽为339 - 371纳米（近紫外线）、435 - 500纳米（蓝色）、510 - 550纳米（绿色）、558 - 636纳米（黄色）和653 - 668纳米（红色）的荧光光源下。在每个实验中，动物暴露于每种光源的同等辐照度下。所使用的不同辐照度分别为0.928、0.200、0.186、0.074和0.019微瓦/平方厘米。所得数据表明，蓝色荧光在抑制松果体褪黑素方面效率最高。发现绿色荧光是抑制松果体褪黑素的次高效光，其次是黄色荧光。近紫外线和红色光抑制松果体褪黑素的能力最弱。这些观察结果表明，在仓鼠中负责介导松果体对光反应的视网膜光色素可能是视紫红质或另一种对蓝光敏感的发色团。
