Hamilton E J, Tostowaryk W, Caron J A, Godard A, Griffiths B W
Clin Biochem. 1984 Feb;17(1):27-30. doi: 10.1016/s0009-9120(84)90405-3.
A reference preparation for human serum proteins (RPHSP) has been developed to aid in the standardization of a wide variety of protein analytes commonly tested by immunodiagnostic procedures. The U.S. national RPHSP was used to cross-calibrate our reference serum by single radial immunodiffusion for the analytes alpha 1-acid glycoprotein, alpha 1-antitrypsin, alpha 2-macroglobulin, ceruloplasmin, C3, C4, haptoglobin, transferrin, IgA, IgG, IgM and by Laurell "rocket" assay for albumin. The statistical evaluation of the cross-calibration study indicates high precision of estimates of the specific proteins assigned to the Canadian RPHSP relative to those of the U.S. reference preparation. Protein concentrations were assigned in both WHO international units (when available) and mass units.
已开发出一种人血清蛋白参考制剂（RPHSP），以帮助对免疫诊断程序中常见检测的多种蛋白质分析物进行标准化。美国国家RPHSP用于通过单向放射免疫扩散法对α1-酸性糖蛋白、α1-抗胰蛋白酶、α2-巨球蛋白、铜蓝蛋白、C3、C4、触珠蛋白、转铁蛋白、IgA、IgG、IgM等分析物以及通过劳雷尔“火箭”法对白蛋白进行交叉校准，从而校准我们的参考血清。交叉校准研究的统计评估表明，相对于美国参考制剂，指定给加拿大RPHSP的特定蛋白质的估计值具有高精度。蛋白质浓度以世界卫生组织国际单位（如有）和质量单位给出。
