Paiement J
Exp Cell Res. 1984 Apr;151(2):354-66. doi: 10.1016/0014-4827(84)90386-0.
Incubation of highly purified nuclei with rough microsomes stripped of associated ribosomes and physiological concentrations of guanosine triphosphate (GTP) led to the fusion of outer membranes of nuclei with microsomes to form large irregular membrane extensions. Measurement of membrane profiles in electron micrographs revealed that the outer membranes of nuclei incubated under these conditions increased significantly in length compared with that of outer membranes of unincubated or control incubated nuclei. This morphometric assay for fusion was used to check membrane and tissue specificity. It was found that GTP did not stimulate fusion between other intracellular membranes (e.g. mitochondrial or Golgi) or between such membranes and nuclear envelopes. GTP did, however, stimulate fusion between stripped rough microsomes from rat liver and outer membranes of nuclei from rat brain. These studies have revealed that membranes of the rough endoplasmic reticulum and nuclear envelope possess unique recognition and fusion properties and as such constitute the first demonstration of membrane interaction specificity at the intracellular level.
将高度纯化的细胞核与去除了相关核糖体的糙面微粒体以及生理浓度的三磷酸鸟苷（GTP）一起温育，导致细胞核的外膜与微粒体融合，形成大的不规则膜延伸物。电子显微镜照片中膜轮廓的测量显示，在这些条件下温育的细胞核的外膜长度与未温育或对照温育的细胞核的外膜相比显著增加。这种融合的形态测定法用于检查膜和组织特异性。发现GTP不会刺激其他细胞内膜（如线粒体或高尔基体）之间或此类膜与核被膜之间的融合。然而，GTP确实会刺激来自大鼠肝脏的去除核糖体的糙面微粒体与来自大鼠大脑的细胞核的外膜之间的融合。这些研究表明，糙面内质网和核被膜的膜具有独特的识别和融合特性，因此构成了细胞内水平膜相互作用特异性的首次证明。
