Kasahara T, Harada H, Ogata K, Kawai T, Shioiri-Nakano K
Microbiol Immunol. 1981;25(12):1345-56. doi: 10.1111/j.1348-0421.1981.tb00143.x.
Significant immunoglobulin (Ig) production by human peripheral blood lymphocytes was induced in vitro by stimulating the cells with pokeweed mitogen (PWM) and Staphylococcus aureus Cowan I (SpA CoI). IgG, IgM, and IgA were determined by a combination of the latex fixation test and radioimmunoassay. High levels (1,000 to 5,000 microgram/ml of IgG and IgM and a lesser amount of IgA were constantly produced during 7 to 8 days of incubation with both stimulants. Ig production induced by SpA CoI stimulation was independent of the presence of T cells, while Ig production induced by PWM required T cells exclusively. Depletion of monocytes in the culture caused but a slight decrease in Ig production (particularly in the case of IgG). While the addition of a small number of monocytes enhanced IgG induction by both stimulants, coculture with an excess number of monocytes inhibited Ig induction (particularly IgG) by PWM stimulation but not by SpA CoI stimulation. Marked suppression of Ig production (IgG, IgM, and IgA) was observed in cocultures with Con A-activated T cells. The phenomena of suppression were observed in both the SpA CoI-stimulated and PWM-stimulated lymphocytes. These data indicated that Ig production from B cells and relatively of independent of monocytes, but could be subjected to the regulation of the Con A-induced suppressor T cells.
用人外周血淋巴细胞体外培养时，通过用美洲商陆有丝分裂原（PWM）和金黄色葡萄球菌Cowan I（SpA CoI）刺激细胞，可诱导产生显著的免疫球蛋白（Ig）。通过乳胶凝集试验和放射免疫测定相结合的方法测定IgG、IgM和IgA。在两种刺激物共同孵育7至8天期间，持续产生高水平（1000至5000微克/毫升）的IgG和IgM，以及少量的IgA。SpA CoI刺激诱导的Ig产生不依赖于T细胞的存在，而PWM诱导的Ig产生仅需要T细胞。培养物中单核细胞的耗竭仅导致Ig产生略有下降（特别是IgG的情况）。虽然添加少量单核细胞可增强两种刺激物诱导的IgG产生，但与过量单核细胞共培养会抑制PWM刺激诱导的Ig产生（特别是IgG），但不抑制SpA CoI刺激诱导的Ig产生。在与Con A激活的T细胞共培养中观察到Ig产生（IgG、IgM和IgA）受到明显抑制。在SpA CoI刺激和PWM刺激的淋巴细胞中均观察到抑制现象。这些数据表明，B细胞产生Ig相对独立于单核细胞，但可受到Con A诱导的抑制性T细胞的调节。
