Perez-Grau L, Bordas J, Koch M H
Nucleic Acids Res. 1984 Mar 26;12(6):2987-96. doi: 10.1093/nar/12.6.2987.
X-ray small angle scattering patterns of solutions and gels of native chicken erythrocyte chromatin and chromatin depleted of H5 histones have been measured under several ionic conditions using synchrotron radiation. Features of the patterns are interpreted as resulting from a superstructure with an outer diameter of about 300A which is already present in uncondensed nucleofilaments. This superstructure which is shown to be maintained by the H5 histones also explains the rapid condensation of the nucleofilaments in higher ionic strengths.
利用同步辐射在几种离子条件下测量了天然鸡红细胞染色质以及去除H5组蛋白的染色质的溶液和凝胶的X射线小角散射图案。图案的特征被解释为是由一种外径约为300埃的超结构产生的,这种超结构在未浓缩的核丝中已经存在。这种由H5组蛋白维持的超结构也解释了在较高离子强度下核丝的快速凝聚。