染色质超结构：溶液和凝胶的同步辐射X射线散射研究
Chromatin superstructure: synchrotron radiation X-ray scattering study on solutions and gels.
作者信息
Perez-Grau L, Bordas J, Koch M H
出版信息
Nucleic Acids Res. 1984 Mar 26;12(6):2987-96. doi: 10.1093/nar/12.6.2987.
Abstract
X-ray small angle scattering patterns of solutions and gels of native chicken erythrocyte chromatin and chromatin depleted of H5 histones have been measured under several ionic conditions using synchrotron radiation. Features of the patterns are interpreted as resulting from a superstructure with an outer diameter of about 300A which is already present in uncondensed nucleofilaments. This superstructure which is shown to be maintained by the H5 histones also explains the rapid condensation of the nucleofilaments in higher ionic strengths.
摘要
利用同步辐射在几种离子条件下测量了天然鸡红细胞染色质以及去除H5组蛋白的染色质的溶液和凝胶的X射线小角散射图案。图案的特征被解释为是由一种外径约为300埃的超结构产生的，这种超结构在未浓缩的核丝中已经存在。这种由H5组蛋白维持的超结构也解释了在较高离子强度下核丝的快速凝聚。
相似文献
1
Chromatin superstructure: synchrotron radiation X-ray scattering study on solutions and gels.染色质超结构：溶液和凝胶的同步辐射X射线散射研究
Nucleic Acids Res. 1984 Mar 26;12(6):2987-96. doi: 10.1093/nar/12.6.2987.
2
The superstructure of chromatin and its condensation mechanism. I. Synchrotron radiation X-ray scattering results.染色质的上层结构及其凝聚机制。I. 同步辐射X射线散射结果。
Eur Biophys J. 1986;13(3):157-73. doi: 10.1007/BF00542560.
3
The superstructure of chromatin and its condensation mechanism. III: Effect of monovalent and divalent cations X-ray solution scattering and hydrodynamic studies.染色质的上层结构及其凝聚机制。III：单价和二价阳离子的影响 X 射线溶液散射和流体动力学研究。
Eur Biophys J. 1987;14(5):307-19. doi: 10.1007/BF00254896.
4
The superstructure of chromatin and its condensation mechanism. IV. Enzymatic digestion, thermal denaturation, effect of netropsin and distamycin.染色质的上层结构及其凝聚机制。IV. 酶消化、热变性、纺锤菌素和偏端霉素的作用
Eur Biophys J. 1987;15(3):133-40. doi: 10.1007/BF00263677.
5
The superstructure of chromatin and its condensation mechanism. II. Theoretical analysis of the X-ray scattering patterns and model calculations.染色质的上层结构及其凝聚机制。II. X射线散射图谱的理论分析与模型计算。
Eur Biophys J. 1986;13(3):175-85. doi: 10.1007/BF00542561.
6
Small angle x-ray scattering of chromatin. Radius and mass per unit length depend on linker length.染色质的小角X射线散射。半径和单位长度质量取决于连接体长度。
Biophys J. 1991 Mar;59(3):606-18. doi: 10.1016/S0006-3495(91)82276-7.
7
The superstructure of chromatin and its condensation mechanism. V. Effect of linker length, condensation by multivalent cations, solubility and electric dichroism properties.染色质的上层结构及其凝聚机制。V. 连接体长度的影响、多价阳离子的凝聚作用、溶解性和电二色性特性
Eur Biophys J. 1988;16(3):177-85. doi: 10.1007/BF00261903.
8
Histone H1 can be removed selectively from chicken erythrocyte chromatin at near physiological conditions.在接近生理条件下，组蛋白H1可以从鸡红细胞染色质中被选择性去除。
Nucleic Acids Res. 1980 Feb 25;8(4):731-9.
9
Protection of discrete DNA fragments by the complex H1-octamerhistones or H5-octamerhistones after micrococcal nuclease digestion.微球菌核酸酶消化后，由H1-八聚体组蛋白或H5-八聚体组蛋白复合物对离散DNA片段的保护作用。
Nucleic Acids Res. 1981 Aug 11;9(15):3671-80. doi: 10.1093/nar/9.15.3671.
10
The superstructure of chromatin and its condensation mechanism. VI. Electric dichroism and model calculations.染色质的上层结构及其凝聚机制。VI. 电二色性与模型计算。
Eur Biophys J. 1989;17(5):245-55. doi: 10.1007/BF00254282.
引用本文的文献
1
Chromatin folding into higher order structure.染色质折叠成更高阶结构。
Biophys J. 1986 Jan;49(1):7-8. doi: 10.1016/S0006-3495(86)83570-6.
2
Three-dimensional structure of extended chromatin fibers as revealed by tapping-mode scanning force microscopy.轻敲模式扫描力显微镜揭示的伸展染色质纤维的三维结构。
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11621-5. doi: 10.1073/pnas.91.24.11621.
3
Viscosity as an additional physico-chemical parameter for studying chromatin structure.粘度作为研究染色质结构的一个额外物理化学参数。
Mol Biol Rep. 1985 Oct;10(4):231-5. doi: 10.1007/BF00775981.
4
The superstructure of chromatin and its condensation mechanism. II. Theoretical analysis of the X-ray scattering patterns and model calculations.染色质的上层结构及其凝聚机制。II. X射线散射图谱的理论分析与模型计算。
Eur Biophys J. 1986;13(3):175-85. doi: 10.1007/BF00542561.
5
The superstructure of chromatin and its condensation mechanism. I. Synchrotron radiation X-ray scattering results.染色质的上层结构及其凝聚机制。I. 同步辐射X射线散射结果。
Eur Biophys J. 1986;13(3):157-73. doi: 10.1007/BF00542560.
6
Chromatin fibers are left-handed double helices with diameter and mass per unit length that depend on linker length.染色质纤维是左手双螺旋结构，其直径和单位长度质量取决于连接体长度。
Biophys J. 1986 Jan;49(1):233-48. doi: 10.1016/S0006-3495(86)83637-2.
7
Small angle scattering of cell nuclei.细胞核的小角散射
Eur Biophys J. 1986;13(6):367-72. doi: 10.1007/BF00265672.
8
The superstructure of chromatin and its condensation mechanism. III: Effect of monovalent and divalent cations X-ray solution scattering and hydrodynamic studies.染色质的上层结构及其凝聚机制。III：单价和二价阳离子的影响 X 射线溶液散射和流体动力学研究。
Eur Biophys J. 1987;14(5):307-19. doi: 10.1007/BF00254896.
9
Chromatin higher-order structure studied by neutron scattering and scanning transmission electron microscopy.通过中子散射和扫描透射电子显微镜研究染色质的高级结构。
Proc Natl Acad Sci U S A. 1987 Nov;84(22):7802-6. doi: 10.1073/pnas.84.22.7802.
10
The superstructure of chromatin and its condensation mechanism. V. Effect of linker length, condensation by multivalent cations, solubility and electric dichroism properties.染色质的上层结构及其凝聚机制。V. 连接体长度的影响、多价阳离子的凝聚作用、溶解性和电二色性特性
Eur Biophys J. 1988;16(3):177-85. doi: 10.1007/BF00261903.
本文引用的文献
1
Preparation of native chromatin and damage caused by shearing.制备天然染色质和剪切引起的损伤。
Science. 1975 Mar 28;187(4182):1203-6. doi: 10.1126/science.187.4182.1203.
2
The higher order structure of chicken erythrocyte chromosomes in vivo.鸡红细胞染色体在体内的高级结构。
Nature. 1980 Dec 11;288(5791):620-2. doi: 10.1038/288620a0.
3
X-ray diffraction study of a new crystal form of the nucleosome core showing higher resolution.对具有更高分辨率的核小体核心新晶体形式的X射线衍射研究。
J Mol Biol. 1981 Feb 5;145(4):757-69. doi: 10.1016/0022-2836(81)90313-2.
4
Nucleosome structure.核小体结构。
Annu Rev Biochem. 1980;49:1115-56. doi: 10.1146/annurev.bi.49.070180.005343.
5
Low angle x-ray diffraction studies of HeLa metaphase chromosomes: effects of histone phosphorylation and chromosome isolation procedure.HeLa中期染色体的低角度X射线衍射研究：组蛋白磷酸化和染色体分离程序的影响
J Cell Biol. 1983 Apr;96(4):1132-7. doi: 10.1083/jcb.96.4.1132.
6
Supranucleosomal organization of chromatin. Electron microscopic visualization of long polynucleosomal chains.染色质的超核小体组织。长多聚核小体链的电子显微镜观察。
Chromosoma. 1982;85(2):251-60. doi: 10.1007/BF00294969.
7
A high-resolution electron microscopy study of nucleosomes from simian virus 40 chromatin.对猿猴病毒40染色质核小体的高分辨率电子显微镜研究。
J Mol Biol. 1981 Jul 15;149(4):735-44. doi: 10.1016/0022-2836(81)90355-7.
8
The molecular structure of nucleohistone (DNH).核组蛋白（DNH）的分子结构。
Exp Cell Res. 1970 Sep;62(1):184-96. doi: 10.1016/0014-4827(79)90519-6.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
Biochem J. 1967 Jan;102(1):251-7. doi: 10.1042/bj1020251.
Zotero 插件