Simon T L, Sierra E R
Transfusion. 1984 Mar-Apr;24(2):173-5. doi: 10.1046/j.1537-2995.1984.24284173355.x.
To determine the best procedure for concentrating platelets in a smaller volume after storage, we studied platelet loss after concentration at various centrifugation g forces for various times. In vitro studies demonstrated the need to let platelets sit for 1 hour prior to resuspension by gentle kneading. 500 X g gave unacceptable results. Losses were minimal at 1500 X g for 7 minutes, 2000 X g for 10 minutes and 5000 X g for 6 minutes. Reinfusion of 51 Cr-labeled platelets into normal volunteers after concentration at both 2000 X g for 10 minutes and 5000 X g for 6 minutes showed normal viability. Numbers and viability of platelets stored up to 5 days in 50 ml plasma and then concentrated in 10 ml plasma after centrifugation at 1500 X g for 7 minutes, 2000 X g for 10 minutes or 5000 X g for 6 minutes should be clinically acceptable.
为确定储存后将血小板浓缩至较小体积的最佳方法，我们研究了在不同离心重力下不同时间进行浓缩后血小板的损失情况。体外研究表明，在通过轻柔揉搓进行重悬之前，需要让血小板静置1小时。500×g的离心力得到的结果不可接受。在1500×g离心7分钟、2000×g离心10分钟和5000×g离心6分钟时，损失最小。在分别以2000×g离心10分钟和5000×g离心6分钟进行浓缩后，将51Cr标记的血小板回输到正常志愿者体内，显示出正常的活力。在50ml血浆中储存长达5天，然后分别以1500×g离心7分钟、2000×g离心10分钟或5000×g离心6分钟在10ml血浆中进行浓缩，血小板的数量和活力在临床上应是可接受的。
