Demidenko O, Tsvetkova S
Histochemistry. 1978 Sep 15;57(3):265-71. doi: 10.1007/BF00492086.
Isolated nuclei of rat hepatocytes were incubated with 0.05% sodium heparinate for 2 to 10 min. Alterations in the nuclei were controlled biochemically, determining the amounts of DNA and histones, and by cytophotometric methods determining the amounts of total and nonhistone proteins and DNA. Under the selected experimental conditions 95% of histones are bound already after 5-min incubation with heparin; nonhistone proteins of cell nuclei remain unchanged. The blockade of histones is followed by DNA diffusion into the incubation medium. Experiments with nuclear staining with alcian blue proved the specificity of heparin binding with histones and showed that heparin-histone complex remains in the nuclei, and its histones lose their extractability with 0.25 n HCl.
将大鼠肝细胞的分离细胞核与0.05%的肝素钠孵育2至10分钟。通过生化方法测定DNA和组蛋白的含量，并通过细胞光度法测定总蛋白、非组蛋白和DNA的含量，从而对细胞核的变化进行控制。在选定的实验条件下，与肝素孵育5分钟后，95%的组蛋白已被结合；细胞核中的非组蛋白保持不变。组蛋白被阻断后，DNA扩散到孵育培养基中。用阿尔辛蓝对细胞核进行染色的实验证明了肝素与组蛋白结合的特异性，并表明肝素-组蛋白复合物保留在细胞核中，其组蛋白在用0.25 N HCl提取时丧失了可提取性。
