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用薄层和高效薄层色谱法对动物组织中的雌激素和抗甲状腺药物进行定量检测(作者译)

[The quantitative detection of estrogens and antithyroid drugs by thin-layer and high performance thin-layer chromatography in animal tissue (author's transl)].

作者信息

Jarc H, Ruttner O, Krocza W

出版信息

J Chromatogr. 1977 Apr 11;134(2):351-8. doi: 10.1016/s0021-9673(00)88533-7.

Abstract

Methods of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) were developed for the determination of estrogens and antithyroid drugs in extracts of animal tissues. TLC proved to be suitable for quantities in the range of 200-2000 ng, with a detection limit of 50-200 ng, HPTLC in the range of 10-200 ng, showing calibration curves of good linearity even in extracts. By HPTLC better detection limits, better separation and faster ascending could be achieved than by TLC.

摘要

开发了薄层色谱法(TLC)和高效薄层色谱法(HPTLC),用于测定动物组织提取物中的雌激素和抗甲状腺药物。TLC适用于200 - 2000 ng范围内的量,检测限为50 - 200 ng;HPTLC适用于10 - 200 ng范围内的量,即使在提取物中也显示出良好的线性校准曲线。与TLC相比,HPTLC可实现更好的检测限、更好的分离效果和更快的上行速度。

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