Yang D C, Dang C V, Arnett F C
Biochem Biophys Res Commun. 1984 Apr 16;120(1):15-21. doi: 10.1016/0006-291x(84)91407-4.
Myositis is an autoimmune inflammatory muscle disease of unknown etiology. We demonstrate directly that the antigen to the myositis-specific anti-Jo-1 antibody is histidyl-tRNA synthetase. The anti-Jo-1 antibody inhibits human HeLa and rat liver histidyl-tRNA synthetase. Using conventional and immunoaffinity chromatography with immobilized anti-Jo-1 antibody, we have purified rat liver histidyl-tRNA synthetase which has a subunit Mr 64,000 and an estimated native Mr suggesting an alpha 2 structure. The evidence indicates that the Jo-1 antigen is histidyl-tRNA synthetase, and that some of the histidyl-tRNA synthetase structure are conserved across species.
肌炎是一种病因不明的自身免疫性炎性肌肉疾病。我们直接证明了肌炎特异性抗Jo-1抗体的抗原是组氨酰-tRNA合成酶。抗Jo-1抗体可抑制人HeLa细胞和大鼠肝脏的组氨酰-tRNA合成酶。通过使用固定化抗Jo-1抗体的常规和免疫亲和层析，我们纯化了大鼠肝脏组氨酰-tRNA合成酶，其亚基分子量为64,000，估计天然分子量表明其为α2结构。证据表明Jo-1抗原是组氨酰-tRNA合成酶，并且组氨酰-tRNA合成酶的一些结构在不同物种间是保守的。
