Walker E J, Tymms K E, Webb J, Jeffrey P D
J Immunol Methods. 1987 Feb 11;96(2):149-56. doi: 10.1016/0022-1759(87)90308-5.
The increased detection of anti-Jo-1 antibody afforded by the use of the purified antigen, histidyl-tRNA synthetase, in counterimmunoelectrophoresis is demonstrated. Using purified antigen, anti-Jo-1 antibody was detected in the sera of 16/33 (48.5%) patients with confirmed myositis and in 20/45 (44.5%) patients with confirmed or possible myositis. This rate is approximately double that obtained with commercial thymus extracts both in this study and seven others reported in the literature. The presence of antibody shows marked correlation with the activity of myositis at the time of serum sampling and with the presence of interstitial lung disease. Detection rates are similar in patients with polymyositis and dermatomyositis both with and without additional connective tissue diseases.
通过在对流免疫电泳中使用纯化抗原组氨酰 - tRNA合成酶，抗Jo - 1抗体的检测率得以提高，这一点得到了证实。使用纯化抗原，在33例确诊肌炎患者的16例（48.5%）血清以及45例确诊或可能患有肌炎患者的20例（44.5%）血清中检测到了抗Jo - 1抗体。在本研究以及文献报道的其他七项研究中，该比率约为使用商业胸腺提取物所获比率的两倍。抗体的存在与血清采样时肌炎的活动度以及间质性肺病的存在显著相关。在伴有或不伴有其他结缔组织病的多发性肌炎和皮肌炎患者中，检测率相似。
