Mathews M B, Bernstein R M
Nature. 1983;304(5922):177-9. doi: 10.1038/304177a0.
In autoimmune disorders such as rheumatoid arthritis and systemic lupus erythematosus (SLE), autoantibodies are generated against a variety of macromolecules. Myositis is a human autoimmune disease characterized by weakness and wasting of muscle. In American studies, antibodies directed against soluble cellular constituents were detected by immunodiffusion in about 60% of cases; the commonest of these, found in 25% of patients, was antibody to the Jo-1 antigen. An antibody system referred to as PL-1 was recognized at a similar frequency in a series of patients studied at Hammersmith Hospital, London. We show here that this system is identical with the Jo-1 system and demonstrate that the antigen is a polypeptide of molecular weight (Mr) 50,000. The protein is immunoprecipitated with tRNA His and appears to be histidyl-tRNA synthetase. The identity of the Jo-1 antigen, the first of the RNA-associated antigens familiar in autoimmune disease to be characterized as a specific enzyme, suggests a model for virus involvement in autoantibody generation.
在类风湿性关节炎和系统性红斑狼疮（SLE）等自身免疫性疾病中，会产生针对多种大分子的自身抗体。肌炎是一种以肌肉无力和萎缩为特征的人类自身免疫性疾病。在美国的研究中，通过免疫扩散在约60%的病例中检测到针对可溶性细胞成分的抗体；其中最常见的是抗Jo-1抗原的抗体，在25%的患者中发现。在伦敦哈默史密斯医院研究的一系列患者中，以类似频率识别出一种称为PL-1的抗体系统。我们在此表明该系统与Jo-1系统相同，并证明该抗原是一种分子量（Mr）为50,000的多肽。该蛋白质与tRNA His免疫沉淀，似乎是组氨酰-tRNA合成酶。Jo-1抗原是自身免疫性疾病中第一个被鉴定为特定酶的与RNA相关的抗原，这提示了病毒参与自身抗体产生的一种模式。
